| 查看: 855 | 回复: 1 | ||
多浩至尊木虫 (知名作家)
|
[求助]
电化学检测限求助
|
|
各位大神好,请帮忙分析一下关于检测限的问题. 如图所示为DPV图和拟合后的图 Methylene blue can adsorb on ss-DNA and increase its concentration whereas the part of the DNA probe involved in hybridization near the detection limit is very low so that higher number of binding sites would result in lower part of the DNA probe converted to ds-DNA and hence in lower relative decay of the Methylene blue signal. The calculations of LOD could confirm the authors suggestion but they did not take into account the standard deviation of the signal. It was determined as 3% for upper quantification limit of target DNA. This means that at least 10% decay of the Methylene blue signal (S/N=3) would correspond to LOD. As could be seen from the Fig. 8 and Fig. 9, 2 microA shift of the signals corresponds to the target DNA concentration of approx. 10-16 mol/L, 100 times higher than LOD declared. The error bars shown in Fig.9A are much lower than those expected from the S.D. announced. The authors did not provide the description of the Methylene blue signal in blank experiments and this complicates the assessment of the metrological characteristics of the DNA sensor. 请问有没有大神指点一下审稿人说的是什么意思? 小弟金币还算多,如能详细解释金币不在话下'再次感谢 |
» 本帖附件资源列表
-
欢迎监督和反馈:小木虫仅提供交流平台,不对该内容负责。
本内容由用户自主发布,如果其内容涉及到知识产权问题,其责任在于用户本人,如对版权有异议,请联系邮箱:xiaomuchong@tal.com - 附件 1 : DPV.tif
- 附件 2 : DPV_linear_relationship.tif
2018-07-22 17:44:17, 79.64 K
2018-07-22 17:44:25, 45.37 K
» 猜你喜欢
德国Karlsruhe Institute of Technology招收电化学储能及联合培养CSC博士等
已经有20人回复
德国Karlsruhe Institute of Technology招收电化学储能及联合培养CSC博士
已经有24人回复
分析化学论文润色/翻译怎么收费?
已经有227人回复
德国Karlsruhe Institute of Technology招收电化学储能及联合培养CSC博士
已经有1人回复
固态锂金属电池迁移数计算求助
已经有1人回复
荷兰奈梅亨大学赵文博与Galimberti课题组招收理论计算CSC博士 2000 欧元/月+房补
已经有0人回复
26储能博士申请自荐
已经有23人回复
上海交通大学--宁波东方理工大学电池方向博士招生
已经有10人回复
海南大学全国重点实验室杨金霖老师招收储能电池方向博士生(2026年3月报名,9月入学)
已经有1人回复
海南师范大学2026年博士研究生招收 (在职想提升学历人员可报考) 申请考核制+普通招考
已经有1人回复
申博求助
已经有0人回复
2楼2018-08-24 23:18:49













回复此楼