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chengh0656银虫 (正式写手)
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[求助]
求大神翻译语一段
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求大神翻译， Enzyme-linkedi mmunosorbenta ssay (ELISA). The ELISAu sed in this study has been describedp reviously (20, 26). Briefly, 96-well flat-bottom plates (Immuno-plate Maxisorp, Nunc, Denmark) were coated with rabbit antiserumi n carbonate buffer (pH 9.6) and incubated at 4 C overnight. The plates were then washed with phosphateb uffer (pH 7.4) containing 0 .05% Tween 20 and blockedw ith 1 % bovine serum albumini n carbonate buffer at 37 C for 1 hr. After washing the plates, six serial 2-fold dilutions of the intestinal contents were made with a phosphate buffer (pH 7.4) containing 1% bovine serum albumin and 0.05% Tween 20. Aliquots (100 IA) of the dilutions were added to each well and incubated at 37 C for 45 min. Detection was carried out with sheep anti-mouse IgG conjugated with horseradish peroxidase (Caltag Laboratories, San Francisco, Calif., U.S.A.), using o-phenylene diamine (Wako Pure Chemical Industries, Tokyo) as the substrate. The plates were read with an EIA reader (Model 2550, Bio-Rad Laboratories, Richmond, Calif, U.S.A.) at 492 nm. The number of CBM588 vegetative cells were determined by interpolation from a standard curve. |
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