| 查看: 793 | 回复: 4 | |||
| 当前主题已经存档。 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
[交流]
紧急求助什么是Lambda DNA？？？
|
|||
| 紧急求助什么是Lambda DNA？？？ |
回复此楼» 猜你喜欢
Fe3O4@SiO2合成
已经有7人回复
-
多组分精馏求助
已经有6人回复
-
免疫学博士有名额，速联系
已经有8人回复
-
26申博求博导推荐-遥感图像处理方向
已经有5人回复
-
国家级人才课题组招收2026年入学博士
已经有6人回复
-
交叉科学部支持青年基金，对三无青椒是个机会吗？
已经有7人回复
-
青椒八年已不青，大家都被折磨成啥样了？
已经有15人回复
-
国家基金申请书模板内插入图片不可调整大小?
已经有6人回复
4楼2009-03-24 11:04:26
2楼2009-03-24 10:39:25
winter_gates
金虫 (正式写手)
生命过客
- 应助: 1 (幼儿园)
- 金币: 1161.8
- 散金: 100
- 红花: 1
- 帖子: 615
- 在线: 20.4小时
- 虫号: 541629
- 注册: 2008-04-08
- 专业: 生物化学
|
Lambda is a temperate Escherichia coli bacteriophage. Virion DNA is linear and double-stranded (48502 nt) with 12 bp single-stranded complementary 5'-ends. After the phage particle injects its chromosome into the cell, the chromosome circularizes by end joining. In the lytic pathway, phage genes encoding replication, lysis and virion proteins are expressed. The chromosome replicates and the replicas are cleaved and packaged into progeny phage particles. In the lysogenic pathway, phage gene expression is repressed, and the circular chromosome inserts into the bacterial chromosome by recombination. Phage lambda DNA is a common substrate for restriction enzymes and for generating DNA size marker fragments. For large scale isolation of phage DNA, cI857Sam7, a mutant carrying four known mutations, is very often in use. The DNA sequence used to construct a phage lambda restriction map includes these mutations. The map shows enzymes that cut lambda DNA once. Enzymes produced by Fermentas are shown in blue. Enzymes which cut lambda DNA once: ApaI 10086, BplI 41036, Bsp120I 10086, Eco105I 12188, EheI 45679, NheI 34679, PdiI 20040, Pfl23II 19323, SanDI 28797, XbaI 24508, XhoI 33498. |
3楼2009-03-24 10:50:15
5楼2009-03-24 11:04:57