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695木虫 (职业作家)
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[交流]
大家帮我看看这几个引物设计的怎么样，刚接触，谢谢
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准备做RT(反转录）-PCR，刚接触，请过来人帮我看看，这几个引物设计的怎么样，多谢了！ 1、 1CTCATAAGGCACCTCCGGTAAACCAAGCCGCCTTGCCGCTGGTAACCAAACCTCGAGCAG >>>>>>>>>>>>>>>>>>>> 61 TGTCCATATCCGTACCCATTACCAGCATAGTCGTTCCAGTACCGGTTTCAATTCCGATTC 121 CAGATTCAACGCCGCCATCTGTAGAGCTTGATGACTCCATAGTTGCCCCATGGGACTACC 181 AATTCTTATTCATGTCACAACGATCGGAAACCACTCAACCTATCACCCTACGTGTTGTGG 241 ATGGTGCAGTACCGGCCGATTTTCCGACCGGTACATACTATCTAACCGGACCTGGGATTC 301TCTCCGACGATCACGGATCGACGGTGCATCCCTTGGACGGCCATGGATACCTAAGAGCAT 361 TCACTTTTGATAATAATGTTACTAACAAAGTCAAATACATGGCTAAGTACATTAGAACCG 421 AGGCTCAATTGGAGGAGCATGACCCTAAGACCAACACGTGGAAATTCACTCATAGAGGTC 481 CATTTTCTGTGTTGAAAGGTGGGAAAAAGGTTGGGAATACTAAGGTCATGAAAAATGTGG 541 CTAACACTAGTGTGTTGAAGTGGGGGGAGAAGCTCCTGTGTATGTGGGAAGGTGGTGAAC 601 CATACGAGATGGAATCCGGTACATTGGATACGATCGGGCGATACAACATGATGGACGATT 661 GTGATTTGGATGATCATGAGAACCCCGGTGATCATGATGTTTGGGATGTTGCTGCTAGCT <<<<<<<<<<<<<<<<<<<< OLIGO start len tm gc% any 3' seq LEFT PRIMER 3 20 59.95 50.00 4.00 0.00 CATAAGGCACCTCCGGTAAA RIGHT PRIMER 717 20 60.26 45.00 3.00 0.00 TAGCAGCAACATCCCAAACA SEQUENCE SIZE: 741 INCLUDED REGION SIZE: 741 2、 1 GTCATTCTTTCCCTGCTACTGACCACTATGTTATTGTGCCTGAAATGCCTCTTAGATACT >>>>>>>>>>>>>>>>>>>> 61 GTGCTCAGAATTTACTAAAGGCTGAGCCTACTCCACTGTACAAGTTCCAGTGGCACCCTG 121 AATCCAAAGCTTTTATGCATGTCATGTGCAAGACTAGTGGAAAGATTGTGGCAAGTGTGG 181 AAGTGCCTCTGTTCATCACTTTCCATTTCATAAATGCGTACGAAGAGGAAGATGAAGATG 241 GGAGGGTGACGGCAGTTATTGCTGACTGTTGTGAGCACAATTCAAATACTGACATTCTTG 301AAAGGCTTAGATTACAGAATCTTCGCTCATTCAATGGCCAAGATGTTCTGCCAGATGCTA 361 AGGTTGGTCGGTTCAGAATACCTCTGGATGGGAGTCCAAATGGAACACTGGAACCAGCAT 421TAGACCCAAATGAACATGGGAAAGGCATGGACATGTGCAGCATAAACCCTAACTATTTAG 481 GGAAGAAATACAGATATGCCTATGCTTGTGGAGCAAACCGACCTTGTAACTTTCCCAACA 541CCCTCACCAAGCTTGATTTAGAGTTGAAAAAGGCTAAGACCTGGTATGATGAAGGTGCTG 601 TGCCATCTGAACCATTCTTTGTGGCTCGACCAGGAGCAACAGAGGAAGATGATGGTTAGA <<<<<<<<<<<<<<<<<<<< 661 CTAGCTACTACATTTGAAGCCTGTTCTCTCTAATATTCAGCCCCTTCT OLIGO start len tm gc% any 3' seq (5’-3’) LEFT PRIMER 5 21 59.86 47.62 2.00 2.00 TTCTTTCCCTGCTACTGACCA RIGHT PRIMER 655 21 59.30 47.62 3.00 0.00 CCATCATCTTCCTCTGTTGCT SEQUENCE SIZE: 708 INCLUDED REGION SIZE: 708 3、 1 ATGGTGTTCATGGATTTTGAATGGTTGTTCCAAATTCCAAGTGTCCCTTGGTCTTCAGCA 61 ATGTTTACCTTGCTGGCTACAATAGGAGGATTCTTAGTGTATCTGTATGGGCCCTACTGG 121 GGTGTGAGGAAAGTTCCAGGTCCACCATCTGTGCCTCTGATAGGACACCTTCCCTTGCTG 181 GCTAAGTATGGCCCTGATGTCTTTTCAGTCCTTGCCAAGCAATATGGCCCAATCTACAGA 241 TTTCATATGGGAAGACAACCTCTAATAATCATAGCAGATGCAGAGCTTTGCAAAGAGGCA 301 GGCATCAAAAAATTTAAAGACATTACAAACAGAAGCATTCCCTCTCCCATTTCTGCATCC 361 CCCCTTCACCAAAAGGGTCTCTTCTTCACCAAAGATTCACAATGGTCTACCATGAGAAAC 421 ACCATATTATCACTCTACCAGCCATCACACTTATCCAGATTAGTGCCAACAATGCAGTCA 481 TTTATAGAATCAGCAACTCAAAATCTAGATTCTCAAAATGAAGACTTCATCTTTTCTAAT 541 CTTTCGCTCAGCTTGGCAACTGATGTGATTGGACAAGCAGCATTTGGTGTTGATTTTGGC 601 CTCTCGAGGCCTCAATCAGTGCGTGATGAAAGTGGCAACAAGGAAGTAAGGGGTTCAGGT 661 GCTGGTAATGAAGTATCAGATTTCATTAACCAACACATTTACTCCACAACCCAACTCAAG 721 ATGGACCTATCTGGTTCCTTCTCCATCATACTGGGTTTACTTGTCCCAATTCTCCAGGAG 781 CCATTTAGGCAGATTCTGAAGAGAATTCCAGGCACCATGGACTGGAAAATCGAGTGTACT 841 AACCGAAAACTAAGTGGCCGTCTTGATGAAATTGTAGAGAAGAGAATGAAGGATAAGGTC 901 AGAAGTTCCAAGGACTTCTTGTCACTCATTCTAAATGCAAGAGAATCAAAAACTGTTTCA 961 GAAAATGTGTTCACACCTGACTATATCAGTGCTGTTACTTATGAGCACCTGCTTGCAGGA 1021TCAGCAACCACATCCTTTACCTTGTCTTCCATTGTCTATTTGGTTGCTGGGCATCCAGAA 1081 GTTGAGAAAAAGATGCTTCAAGAGATTGATGGGTTTGGCCCAGTAGATCAGACACCAACT 1141 TCTCAAGATCTTCAAGAGAAGTTTCCTTACCTTGATCAGGTGATCAAAGAGGCCATGAGA 1201 TATTACACAGTCTCTCCATTGGTTGCAAGAGAAACATCAAATGAAGTAGAGATTGGAGGT 1261 TACCTTCTTCCAAAGGGGACTTGGGTCTGGTTAGCGCTTGGAGTTGTAGCAAAAGATCCC 1321 AGAAACTTTCCGGAGCCAGAAAAGTTCAAACCGGAGAGGTTTGATCCCAAATGTGAAGAA 1381 ATGAAAAGAAGACATCCTTATGCTTTCATACCATTTGGAATTGGTCCTCGGGCTTGCATT 1441 GGTCAGAAATTTTCCCTGCAAGAAATCAAGCTGTCTCTGATTCACTTATATAGGAAATAC 1501 TTGTTTCGGCATTCACCTAATATGGAGAATCCCTTAGAACTTGAATATGGCATAGTGCTC 1561 AACTTCAAGCACGGTGTCAAGGTTAGAGCCATAAAAAGAACAGAGAGGAGCTGCTAA OLIGO start len tm gc% any 3' seq(5’-3’) LEFT PRIMER 132 20 59.96 55.00 4.00 2.00 AGTTCCAGGTCCACCATCTG RIGHT PRIMER1578 20 59.88 50.00 5.00 3.00 GACACCGTGCTTGAAGTTGA SEQUENCE SIZE: 1617 INCLUDED REGION SIZE: 1617 4、 1 ATGAGTAACGCTGCTGAAACCACCGTCAGGCACTTGCCGGAGGAGATCCTGTTGAAGGTG 61 TTCTCCGGGGTTAGTGATACCCGGACGCGGAACTCGCTCTCGCTTGTTTGCCGGAGCTTC 121TACTGCTTCGAGCGCAGGACGCGCTCCTCCCTCACGCTCCGGGGAATCGCGCGTGACCTC 181 TACTTGATCCCGACCTGCTTCGCGCACGTCACGCACCTCGACTTGTCGCTCCTCTCGCCG 241 TGGGGCCACGCGCTGCTCTGCTCGTCCTCCACCGCCGACCCTCACCACCTTGCCCAACGC 301CTCCGCGACGCCTTCCCGCGCGTGACCTCGCTCGTCGTCTACGCGCGCGAACCGACCACG 361 CTCCACCTTCTCCTGCTCTCTCCCTGGCCGGAGCTCCGCCATGTTCGCCTTGTCCGATGG 421 CACCAGCGCCCGCCGTCGTCGCCAGCTGGATCTGACTTCGCGACTTTGTTCTCGCGCTGC 481 CGATCGCTCACCTCGCTGGATCTCTCCGCCTTCTACCACTGGCCGGAGGATCTCCCGCCT 541 GTCCTGACAGCGAACCCCGCCGCCGCCGCATCTCTACGGCGGCTGAACCTCCTAAAGACG 601 TCGTTCACGGAGGGCTTCAAGTCTCACGAAATCGAATCCATCACCGCATCTTGCCCTAAC 661 CTGGAGCACTTTCTCGTGGCCTGCACTTTCGATCCGAGATACATAGGGTTCGTCGGTGAC 721 GAGACGCTGTCGGCGATCCCTTCCAATTGCCCCAAGCTCTCGCTCCTTCACCTTGCTGAT 781 ACGTCATCGTTTTCGAACCGGAGCGACGATGACGGTAACGGCGGGGAGGACGCGAGGATT 841 AGCCACGAGACGCTGGTGGCGTTGTTCTCCGGGCTTCCCCTTTTGGAAGAGCTGGTGCTA 901 GATGTTTGTAAAAATGTCAGAGAGAGTAGTTTTGCTATGGAGGTTTTGAGTAAGAAGTGT 961 CCCAATTTGAGGGTTCTCAGGTTGGGTCAGTTTCAGGGGATTTGCTTGGCCATTGGGTCA 1021 AAGCTCGATGGAATTGCCCTTTGCCAAGGGCTTCGATCCTTGTCCATTCATGGCTGTGCG 1081 GACCTCGATGACATGGGGTTGATTGAGATTGCAAGAGGTTGTTCCAGGTTGGTTCAGTTT 1141 GAGCTTCAGGGCTGCAAGCTTGTCACGGAGAAAGGGTTGGGGACCATGGCTTGCTTGCTT 1201 CGTAAGACTCTTGTGGATGTCAAGGTTTCTTGCTGTGTCAATCTCGACACCGCGGCGGCA 1261 CTCAGAGCTCTGGACCCTATCCGGGACCGGATTGAGCGGCTTCATGTGGATTGCGTGTGG 1321 AATGGCTTGAAGGACAGTGATGACAACATGGGAGGTGGTCTTCTCAATTTTGACCTGAAT 1381 GACCCCAATGGTGGTGCTGAAATCATGGATTGCTTTGGGGATGAGGAATGTGAAGACCCA 1441 AGCAAAAGGAAGAGGCAGAGATGCGAGTATGGACTAGAGGGTGATGATTCCTTGGTGCAA 1501 AGCAATGGCAATGGTTACTATGGCAAGAACTGGGACAGGCTGCGTTATCTCTCTCTTTGG 1561 ATAAAGGTTGGTGATCTTCTGAATCTCTTACCGGTGGCAGGGTTGGAGGATTGCCCTAAC 1621 CTGGAAGAGATTCGTGTGAAGGTTGAAGGAGATTGCAGGGGGCAGCCAAAACCAGCTGAG 1681 AGTGAATTTGGTCTCAGCATCCTGGCTTGTTATCCACAGCTATCAAAGATGCAGCTGGAT 1741 TGTGGTGATACAAGAGGCTATGTGCTCACAGCGCCTTCAGGGCAGATGGATCTGAGCTTG 1801 TGGGAGAGGTTTTTTCTCAATGGGATTAGTAGTTTGAGTCTGAATGAGCTTCATTACTGG 1861 CCACCACAAGATGAGGACGTAAACCTGAGGAGTGTTTCACTTCCAGCTGCCGGCTTACTT 1921 CAAGAATGTTACACCCTGAGGAAGCTTATCATTCATGGGACAGCTCATGAACATTTCATG 1981 AATTTTTTCCTCAAGATCCCAAACCTGAGGGATGTGCAGCTGCGAGAGGATTATTATCCA 2041 GCACCGGCAAGTGACATGAGCACGGAGATCAGGGTGGGTTCATGCAGCCGCTTTGAAGAT 2101 GCATTAAACAGGCGTCATATCTGTGATTGA OLIGO start len tm gc% any 3' seq LEFT PRIMER106 20 60.02 55.00 4.00 1.00 GTTTGCCGGAGCTTCTACTG RIGHT PRIMER1810 20 59.99 55.00 4.00 3.00 ACCTCTCCCACAAGCTCAGA SEQUENCE SIZE: 2130 INCLUDED REGION SIZE: 2130 |
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