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Cloning, sequencing, and expression of an endoglucanase gene from the rumen anaerobic fungus Neocallimastix frontalis MCH3. Fujino Y, Ogata K, Nagamine T, Ushida K. Laboratory of Animal Science, Kyoto Prefectural University, Kyoto, Japan. A cDNA clone encoding an endo-1,4-beta-glucanase from a rumen fungus, Neocallimastix frontalis MCH3, was isolated. The nucleotide sequence showed that the gene, celA, encoded a multidomain enzyme containing a family 5 catalytic domain and a reiterated sequence that is involved in the association of a multienzyme complex, the cellulosome. The enzyme expressed in Escherichia coli showed the highest activity against carboxymethylcellulose at 40 degrees C and pH 8.5. [ Last edited by 王雨云 on 2009-4-16 at 09:33 ] |
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王雨云(金币+1,VIP+0):虽然我早就下载到了，但是还是谢谢你！ 4-16 09:35
王雨云(金币+1,VIP+0):虽然我早就下载到了，但是还是谢谢你！ 4-16 09:35
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