| 查看: 171 | 回复: 1 | |||
| 当前主题已经存档。 | |||
ashan631铁虫 (小有名气)
|
[交流]
[交流/求助]一篇succinate dehydrogenase和fumarate reductase的文献，小虫感激万分
|
||
|
[Title] Partial replacement of succinate dehydrogenase function by phage- and plasmid-specified fumarate reductase in Escherichia coli [Author] Guest JR [Abstract] Phages capable of transducing succinate dehydrogenase mutants (sdh) of Escherichia coli were isolated from pools of artificially constructed recombinant lambda phages using a selective casein digest medium. These phages produced characteristically dense turbid plaques, and as prophages they increased the aerobic growth efficiencies of sdh mutants on complex media but were unable to promote growth with succinate as sole carbon and energy source (an essential feature of sdh + strains). The phages were identified as fumarate reductase transducing phages (lambda frdA) by the presence of a characteristic 4.9 kilobase pairs R.HindIII fragment of bacterial DNA, the expression of a polypeptide with a relative molecular mass of 72,000 (the frdA gene product) and by comparing their transducing activities with authentic lambda frdA phages. In parallel studies a strain containing a ColE1-frd hybrid plasmid (pGS1 = pLC16.43) was characterized. Transfer of pGS1 to sdh mutants was accompanied by increased aerobic growth efficiencies on complex media and the ability to utilize succinate as sole carbon and energy source. It was concluded that fumarate reductase can replace succinate dehydrogenase but the extent of the reversal of the sdh lesion depends on frd gene dosage and the titration of the repressor which normally prevents aerobic synthesis of the reductase. Best wishes. |
回复此楼» 猜你喜欢
售SCI一区T0P文章，我:8.O.55.1.O.5.4,科目全,可+急
已经有6人回复
-
售SCI文章，我:8O5.5.1.O.54,科目齐全,可+急
已经有6人回复
-
【长期招聘】复旦大学 AI 制药实验室 博士后招聘
已经有11人回复
-
售SCI一区T0P文章，我:8.O.55.1.O.5.4,科目全,可+急
已经有7人回复
-
2027博士申请-临床检验诊断学、病原生物学、微生物方向
已经有5人回复
-
各位基础老师们，看完中央《从严治党》的决定后，对学术界更加有信心了吧
已经有4人回复
ashan631
铁虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 2271.5
- 散金: 37
- 帖子: 111
- 在线: 52.2小时
- 虫号: 496449
- 注册: 2008-01-18
- 专业: 微生物生理与生物化学
2楼2009-10-21 19:44:15