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lenaimiao

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[交流] 如何看A260/A280

用试剂盒提细胞DNA,两个样,测A260/A280均在1.91—1.94,,A260/A230均大于2,在2.1左右。求指教这个提的质量怎样

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yubeihong

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2楼2018-01-19 14:21:50
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zhouxfd

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Nucleic acids and proteins have absorbance maxima at
260 and 280 nm, respectively. Historically, the ratio of
absorbances at these wavelengths has been used as a
measure of purity in both nucleic acid and protein
extractions. A ratio of ~1.8 is generally accepted as
“pure” for DNA; a ratio of ~2.0 is generally accepted as
“pure” for RNA.
Similarly, absorbance at 230 nm is accepted as being the
result of other contamination; therefore the ratio of A260/
A230 is frequently also calculated. The 260/230 values for
“pure” nucleic acid are often higher than the respective
260/280 values. Expected 260/230 values are commonly
in the range of 2.0–2.2.
3楼2018-01-20 09:01:20
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