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# Daily English Learning of Analytical Instrument # Topic: High-Performance Liquid Chromatography(HPLC)-2 ¿ÆÑÐÒÇÆ÷Ó¢Óï¿ÚÓïÿÈÕÒ»Á·¡ª¡ªÒºÏàÉ«Æ×£¨¶þ£© This is oral English of analytical instrument organized by EWG1990 English group. Welcome to lesson 5. Today we continue with the second part of High-Performance Liquid Chromatography£¨HPLC£© Now let's begin the part 1£º ☞ Vocabularies: 1. ÖùÀÏ»¯ condition (aging) of column 2. ÖùÁ÷ʧ column bleeding 3. ÖùÎÂÏä column oven 4. ×î¼ÑÁ÷ËÙ optimum flow rate 5. Ïà¶Ô±£Áôʱ¼ä relative retention time 6. ÑôÀë×Ó½»»»¼Á cation exchanger 7. ÉýÎÂËÙÂÊ temperature rate 8. ·åÃæ»ý peak area 9. ËÀÌå»ý dead volume 10. Á÷¶¯Ïà mobile phase 11. ¹è½º silica gel 12. ³ÌÐòÉýΠprogrammed temperature Now let's begin the part 2£º ☞ Sentences: 1. ÝÍÈ¡¹ý³Ì°üÀ¨ÖùÀÏ»¯¡¢ÉÏÑù¡¢Ï´µÓ¡¢Ï´ÍÑÒÔ¼°ÖùÔÙÉúµÈ²½Öè¡£ The extraction procedure included conditioning, loading, washing, eluting and regenerating. 2. ¶ÔËù½¨Á¢µÄʵÑé·½·¨½øÐп¼²ì£¬¸÷É«Æ×·åÏà¶Ô±£Áôʱ¼äµÄÏà¶Ô±ê׼ƫ²îRSD£¼0.03 %£¬Ïà¶Ô·åÃæ»ýµÄÏà¶Ô±ê׼ƫ²îRSD£¼5.0 %¡£ The established methodology was investigated and RSD of the relative retention time for each chromatographic peak was less than 0.03% and RSD of relative peak area was less than 5.0%. 3. Ñо¿½á¹û±íÃ÷£¬Î¶ȱ仯ÂʺÍζÈÌݶȵÄÓ°Ïì²»ÈݺöÊÓ¡£ The results show that effects of temperature rate and its gradients are not invisible. 4. ±¾´ÎʵÑéµÄ²»È·¶¨¶ÈÖ÷ÒªÓɹ©ÊÔÆ·ÈÜÒº·åÃæ»ýµÄÖØ¸´ÐÔ²âÁ¿ÒýÆðµÄ¡£ The uncertainty of the experiment is mainly caused by duplicated measurement of peak area of the test solution. 5. ¹è½ºÖùÉ«Æ×ºÍÄý½ºÖùÉ«Æ×µÄ»ìºÏÀûÓÿÉÒÔÌá¸ß¹©ÊÔÆ·µÄ·ÖÀë´¿»¯Ð§¹û¡£ The co-utilization of silica gel column and gel chromatography can greatly improve the efficiency of isolation and purification. This is the end of the class,please have a wonderful day! Ïë»ñÈ¡ÔÎÄÓ¢ÓïÒôƵ£¬ÇëÒÆ²½https://muchong.com/t-11968611-1 |
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