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# Daily English Learning of Analytical Instrument #

Topic:  High-Performance Liquid Chromatography(HPLC)-2

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This is oral English of analytical instrument organized by EWG1990 English group. Welcome to lesson 5. Today we continue with the second part of High-Performance Liquid Chromatography£¨HPLC£©

Now let's begin the part 1£º

☞ Vocabularies:

1. ÖùÀÏ»¯ condition (aging) of column
2. ÖùÁ÷ʧ column bleeding
3. ÖùÎÂÏä column oven
4. ×î¼ÑÁ÷ËÙ optimum flow rate
5. Ïà¶Ô±£Áôʱ¼ä relative retention time
6. ÑôÀë×Ó½»»»¼Á cation exchanger
7. ÉýÎÂËÙÂÊ temperature rate
8. ·åÃæ»ý peak area
9. ËÀÌå»ý dead volume
10. Á÷¶¯Ïà mobile phase
11. ¹è½º silica gel
12. ³ÌÐòÉýΠprogrammed temperature

Now let's begin the part 2£º

☞ Sentences:

1. ÝÍÈ¡¹ý³Ì°üÀ¨ÖùÀÏ»¯¡¢ÉÏÑù¡¢Ï´µÓ¡¢Ï´ÍÑÒÔ¼°ÖùÔÙÉúµÈ²½Öè¡£
The extraction procedure included conditioning, loading, washing, eluting and regenerating.

2. ¶ÔËù½¨Á¢µÄʵÑé·½·¨½øÐп¼²ì£¬¸÷É«Æ×·åÏà¶Ô±£Áôʱ¼äµÄÏà¶Ô±ê׼ƫ²îRSD£¼0.03 %£¬Ïà¶Ô·åÃæ»ýµÄÏà¶Ô±ê׼ƫ²îRSD£¼5.0 %¡£
The established methodology was investigated and RSD of the relative retention time for each chromatographic peak was less than 0.03% and RSD of relative peak area was less than 5.0%.

3. Ñо¿½á¹û±íÃ÷£¬Î¶ȱ仯ÂʺÍζÈÌݶȵÄÓ°Ïì²»ÈݺöÊÓ¡£
The results show that effects of temperature rate and its gradients are not invisible.

4. ±¾´ÎʵÑéµÄ²»È·¶¨¶ÈÖ÷ÒªÓɹ©ÊÔÆ·ÈÜÒº·åÃæ»ýµÄÖØ¸´ÐÔ²âÁ¿ÒýÆðµÄ¡£
The uncertainty of the experiment is mainly caused by duplicated measurement of peak area of the test solution.

5. ¹è½ºÖùÉ«Æ×ºÍÄý½ºÖùÉ«Æ×µÄ»ìºÏÀûÓÿÉÒÔÌá¸ß¹©ÊÔÆ·µÄ·ÖÀë´¿»¯Ð§¹û¡£
The co-utilization of silica gel column and gel chromatography can greatly improve the efficiency of isolation and purification.

This is the end of the class,please have a wonderful day!

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