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gzdeng

铁杆木虫 (著名写手)

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[交流] 求助，请帮忙翻译

由于没有生物背景，现需要翻译下面内容，怕翻译不准，请兄弟姐妹们帮帮忙，非常感谢！
DNA templates were prepared　by the freeze-thaw method in a DNA extraction buffer(25). PCR was conducted for 40 cycles, with denaturation　at 94°C for 60 s, annealing for 30 s, and extension　at 72°C for 30s using the PROGRAM TEMP CONTROL　SYSTEM (PC-800, ASTEC, Fukuoka). Here,　considering that each Cl20 or C230 target sequence has　a different homology score with the designed primers, a　step down method was employed (26). For the Cl20　genes, the annealing temperature was 61’C in the first 10　cycles followed by a step down to 59°C in the next 15　cycles, and 57°C in the last 15 cycles, while for the C230　genes, the annealing temperature was 59°C in the first 10　cycles, 57°C in the next 15 cycles, and 55°C in the last　15 cycles. Aliquots (10~1) of the PCR products were　analyzed by electrophoresis on a 1.0% agarose gel stained　with 0.5 pg/ml ethidium bromide.

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