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±¾ÈË·ÒëÁËÒ»¶ÎÕªÒª£¬µ«ÊÇˮƽÓÐÏÞ£¬Çë³æÓѰïæÈóɫһÏ£¡ ÖÐÎÄÔÎÄ£º²ÉÓó¬¸ßЧҺÏàÉ«Æ×´®Áª¸ß·Ö±æËļ«¸Ë·ÉÐÐʱ¼äÖÊÆ×·¨£¨UPLC-Q-TOP-MS£©½áºÏ´ó»·ÄÚõ¥À࿹ÉúËØÊý¾Ý¿â£¬½¨Á¢¿ìËÙɸ²é¼ø±ðÖíѪ½¬Öг£¼û´ó»·ÄÚõ¥À࿹ÉúËØµÄ·½·¨¡£ÖíѪ½¬¾¼×´¼ÌáÈ¡£¬Í¨¹ýUPLC-Q-TOP-MSÕýÀë×ÓģʽȫɨÃè·ÖÎö£¬µÃµ½ÖíѪ½¬Öдó»·ÄÚõ¥À࿹ÉúËØ×¼·Ö×ÓÀë×ӷ徫ȷÖÊÁ¿Êý¡¢Ö÷ÒªË鯬·åµÄ¾«È·ÖÊÁ¿ÊýºÍ±£Áôʱ¼ä£¬½áºÏ´ó»·ÄÚõ¥À࿹ÉúËØÊý¾Ý¿âɸ²é£¬ÊµÏÖÖíѪ½¬Öдó»·ÄÚõ¥À࿹ÉúËØµÄ¿ìËÙ¶¨ÐÔʶ±ð¡£Ëù½¨Êý¾Ý¿â°üº¬26ÖÖ³£¼ûÖíѪ½¬Öдó»·ÄÚõ¥À࿹ÉúËØµÄ×¼·Ö×ÓÀë×ӷ徫ȷÖÊÁ¿Êý¡¢Ö÷ÒªË鯬·åµÄ¾«È·ÖÊÁ¿ÊýºÍ±£Áôʱ¼ä£¬ÆäÖÐ13ÖÖ³£¼û´ó»·ÄÚõ¥À࿹ÉúËØµÄ¼ì²âÏÞÔÚ10-70ng/mL·¶Î§ÄÚ¡£¸Ã·½·¨·ÖÀë¶È¸ß¡¢¼ì²âÁéÃô£¬¿ÉÒÔ×÷Ϊ¿ìËÙɸ²é¼ø±ðÖíѪ½¬ÖеĴó»·ÄÚõ¥À࿹ÉúËØµÄÓÐЧ·½·¨¡£ Ó¢ÎÄ·Ò룺A new method was developed for rapid screening and identification of common macrolide antibiotics in swine plasma by ultra-performance liquid chromatography-tandem high resolution quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOP-MS) combined with macrolide antibiotics database. The swine plasma sample was extracted with methanol, and then the extracts were analyzed by UPLC-Q-TOP-MS in positive full scan modes. The accurate-mass of quasi-molecular ion peak, accurate-mass of main fragment ion peaks and etention time of macrolide antibiotics in swine plasma were acquired and the macrolide antibiotics in swine plasma could be successfully qualitative identification by database screening. The created database includes accurate-mass of quasi-molecular ion peak, accurate-mass of main fragment ion peaks and retention time of 26 commom macrolide antibiotics. The limits of detection (LOD) of 13 macrolide antibiotics were in the range of 10-70ng/mL. The results showed that the proposed UPLC-Q-TOP-MS method was sensitive and had the high separation efficiency. The method was a useful tool for the rapid screening and qualitative identification of common macrolide antibiotics in swine plasma. |
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