| 查看: 1836 | 回复: 3 | |||
[交流]
关于NCBI上传序列中遇到的一些问题 已有3人参与
|
|
[1] Please clarify what type of molecule you isolated and sequenced. Are your submissions: - mRNA (cDNA) sequence(s), or - genomic DNA, but [a] intronless region of genomic sequence or noncontiguous genomic sequence(s) (introns removed) [2] Some or all of the protein coding sequences contain reading frame shifts (insertions/deletions based on BLAST similarity search results and/or an alignment), and/or have translations that show little or no similarity to other proteins in the database: Deletion at alignment position 75 Gap: 1 Non-gap: 6 BankIt2063341/R-YL1 Deletion at alignment position 984 Gap: 2 Non-gap: 2 BankIt2063341/R-BX3,BankIt2063341/R-DB2 Alignment attached. This generally indicates problems such as: - errors in the nucleotide sequence - sequences have not been trimmed of low quality - problems with feature annotation which may be due to: translation in the wrong reading frame or on the wrong strand translation does not have the correct exon spans translation includes noncoding sequence, such as introns or UTR's 以上是NCBI回复邮件中提到的问题。 是做真菌分子鉴定时测序得到的序列,几百bp,是一个基因的部分片段,问题主要是出在CDS功能注释这部分,本人对这个东西不太明白,没搞懂应怎样做出修改,请各位大神相助 |
» 猜你喜欢
HILIC磁珠研究蛋白组学怎么做?
已经有0人回复
His标签蛋白纯化怎么才能快?
已经有0人回复
化学工程及工业化学论文润色/翻译怎么收费?
已经有104人回复
WRN变构抑制剂HRO761:从D1/D2界面锁定到MSI合成致死的工具化合物深度解析
已经有0人回复
KRAS G12V(ON) 选择性抑制剂 RMC-5127:从三元复合物机制到科研应用
已经有0人回复
SHP2 变构抑制剂 Sitneprotafib:从作用机制到科研试剂选型要点
已经有0人回复
PRMT5·MTA 抑制剂 MRTX1719 的科研应用
已经有0人回复
SW033291 科研使用解析:分子特征、储存与实验获取要点
已经有0人回复
泛 RAS 抑制新思路:大环肽拟似物如何同时牵住突变型与野生型 RAS?
已经有0人回复
TYK2/JAK1 双靶点工具化合物 Brepocitinib:激酶选择性、研究进展与实验应用全解析
已经有0人回复
矢车菊素-3-O-葡萄糖苷质控实录:从一张HPLC图谱读懂纯度与稳定性
已经有0人回复
» 本主题相关商家推荐: (我也要在这里推广)
2楼2018-04-08 22:02:40
3楼2018-06-25 21:55:09
4楼2018-08-29 20:34:20










回复此楼

