| 查看: 1781 | 回复: 3 | |||
[交流]
关于NCBI上传序列中遇到的一些问题 已有3人参与
|
|
[1] Please clarify what type of molecule you isolated and sequenced. Are your submissions: - mRNA (cDNA) sequence(s), or - genomic DNA, but [a] intronless region of genomic sequence or noncontiguous genomic sequence(s) (introns removed) [2] Some or all of the protein coding sequences contain reading frame shifts (insertions/deletions based on BLAST similarity search results and/or an alignment), and/or have translations that show little or no similarity to other proteins in the database: Deletion at alignment position 75 Gap: 1 Non-gap: 6 BankIt2063341/R-YL1 Deletion at alignment position 984 Gap: 2 Non-gap: 2 BankIt2063341/R-BX3,BankIt2063341/R-DB2 Alignment attached. This generally indicates problems such as: - errors in the nucleotide sequence - sequences have not been trimmed of low quality - problems with feature annotation which may be due to: translation in the wrong reading frame or on the wrong strand translation does not have the correct exon spans translation includes noncoding sequence, such as introns or UTR's 以上是NCBI回复邮件中提到的问题。 是做真菌分子鉴定时测序得到的序列，几百bp，是一个基因的部分片段，问题主要是出在CDS功能注释这部分，本人对这个东西不太明白，没搞懂应怎样做出修改，请各位大神相助 |
回复此楼» 猜你喜欢
含氟糖皮质激素的外用抗炎利器醋酸氟轻松
已经有0人回复
-
阿莫奈韦（Amenamevir）：解旋酶-引物酶靶向分子的结构特征与合成路径全解析
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有289人回复
-
利瑞鲁单抗：靶向KIR的NK细胞检查点工具抗体，结构基础与科研应用全解析
已经有0人回复
-
Wnt-C59：PORCN靶向化学探针，Wnt信号通路阻断机制与科研应用全解析
已经有0人回复
-
Fexaramine：肠道限制性FXR化学探针，结构特征与信号通路研究全解析
已经有0人回复
-
NVS-STG2：靶向STING跨膜域的分子胶类激动剂，结构特征与寡聚化机制研究全解析
已经有0人回复
-
DOTA-GPC3肽（CAS 3066439-94-6）：双功能分子探针前体的结构特征与标记应用解析
已经有0人回复
-
科研用 TLR7/8 双激动剂怎么选？现货与质控要点
已经有0人回复
-
SEL120-34A HCl：CDK8/CDK19高选择性激酶抑制剂
已经有0人回复
» 本主题相关商家推荐: (我也要在这里推广)
2楼2018-04-08 22:02:40
3楼2018-06-25 21:55:09
4楼2018-08-29 20:34:20