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[交流] 关于NCBI上传序列中遇到的一些问题已有3人参与

[1] Please clarify what type of molecule you isolated and sequenced. Are
your submissions:

- mRNA (cDNA) sequence(s), or
- genomic DNA, but
[a] intronless region of genomic sequence
or
noncontiguous genomic sequence(s) (introns removed)

[2] Some or all of the protein coding sequences contain reading frame shifts
(insertions/deletions based on BLAST similarity search results and/or an
alignment), and/or have translations that show little or no similarity
to other proteins in the database:

Deletion at alignment position 75
Gap: 1 Non-gap: 6
BankIt2063341/R-YL1

Deletion at alignment position 984
Gap: 2 Non-gap: 2
BankIt2063341/R-BX3,BankIt2063341/R-DB2

Alignment attached.

This generally indicates problems such as:

  - errors in the nucleotide sequence
  - sequences have not been trimmed of low quality
  - problems with feature annotation which may be due to:
translation in the wrong reading frame or on the wrong strand
translation does not have the correct exon spans
translation includes noncoding sequence, such as introns or UTR's

以上是NCBI回复邮件中提到的问题。
是做真菌分子鉴定时测序得到的序列，几百bp，是一个基因的部分片段，问题主要是出在CDS功能注释这部分，本人对这个东西不太明白，没搞懂应怎样做出修改，请各位大神相助