| 查看: 651 | 回复: 10 | |||
| 当前主题已经存档。 | |||
bioinfuture铁虫 (小有名气)
|
[交流]
求助一篇sciencedirect文章，必有重奖
|
||
|
Evaluation of replicative capacity and genetic stability of West Nile virus replicons using highly efficient packaging cell lines This article is not included in your organization's subscription. However, you may be able to access this article under your organization's agreement with Elsevier. Rafik Fayzulina, Frank Schollea, 1, Olga Petrakovab, Ilya Frolovb and Peter W. Masona, b, c, , aDepartment of Pathology, 3.206B Mary Moody Northen Pavilion, University of Texas Medical Branch, 301 University Boulevard, Galveston, TX 77555-0436, USA bDepartment of Microbiology and Immunology, UTMB, 301 University Boulevard, Galveston, TX 77555, USA cSealy Center for Vaccine Development, UTMB, 301 University Boulevard, Galveston, TX 77555, USA Received 6 February 2006; revised 23 February 2006; accepted 25 February 2006. Available online 2 May 2006. Abstract A stable cell system for high-efficiency packaging of West Nile virus (WNV) subgenomic replicons into virus-like particles (VLPs) was developed. VLPs could be propagated on these packaging cells and produced infectious foci similar to foci produced by WNV. Focus size correlated with the replicative capacity of WNV replicons, indicating that genome copy number, rather than amount of trans-complementing structural proteins, was rate-limiting in packaging of VLPs. Comparison of VLP production from replicon genomes encoding partial or complete C genes indicated that portions of C downstream of the cyclization sequence could improve genome replication or that cis expression of C could enhance packaging. Interestingly, a rapid loss of replicon-encoded reporter gene activity was detected within two serial passages of reporter gene-containing VLPs. The loss of reporter activity correlated with gene deletion and better VLP growth, indicating a powerful selection pressure for WNV genomes lacking reporter genes. Keywords: Flavivirus; Replicon; Packaging; Virus-like particle; Noncytopathic replicon |
回复此楼» 猜你喜欢
化学工程，本211，求调剂，ab区不限
已经有4人回复
-
湖北师范大学招调剂啦
已经有9人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有174人回复
-
邵阳学院食品与化学工程学院硕士调剂
已经有0人回复
-
天津商业大学 生物与医药课题组招生
已经有0人回复
-
生物化工学硕招收调剂
已经有0人回复
-
广东石油化工学院2026年硕士研究生需要多名生物，制药工程，食品专业的调剂生
已经有0人回复
-
317分 一志愿江南大学 化学工程学硕 求调剂
已经有6人回复
-
化工申博
已经有3人回复
-
申博自荐
已经有0人回复
qwj_1001
木虫 (著名写手)
- 应助: 0 (幼儿园)
- 金币: 1982.3
- 散金: 850
- 红花: 6
- 帖子: 1383
- 在线: 141.1小时
- 虫号: 664683
- 注册: 2008-11-29
- 性别: GG
- 专业: 食品加工技术
2楼2009-02-23 08:14:47
liyouran85
金虫 (小有名气)
- 应助: 1 (幼儿园)
- 金币: 690.9
- 红花: 3
- 帖子: 155
- 在线: 127小时
- 虫号: 487083
- 注册: 2007-12-28
- 专业: 微生物生理与生物化学
3楼2009-02-23 09:15:08
liyouran85
金虫 (小有名气)
- 应助: 1 (幼儿园)
- 金币: 690.9
- 红花: 3
- 帖子: 155
- 在线: 127小时
- 虫号: 487083
- 注册: 2007-12-28
- 专业: 微生物生理与生物化学
4楼2009-02-23 09:17:24
qwj_1001
木虫 (著名写手)
- 应助: 0 (幼儿园)
- 金币: 1982.3
- 散金: 850
- 红花: 6
- 帖子: 1383
- 在线: 141.1小时
- 虫号: 664683
- 注册: 2008-11-29
- 性别: GG
- 专业: 食品加工技术
5楼2009-02-23 10:20:33
bioinfuture
铁虫 (小有名气)
- 应助: 0 (幼儿园)
- 金币: 2.8
- 散金: 43
- 帖子: 195
- 在线: 9.2小时
- 虫号: 674534
- 注册: 2008-12-14
- 性别: GG
- 专业: 兽医传染病学
6楼2009-02-23 18:31:25
7楼2009-02-24 10:30:01
8楼2009-02-24 15:05:52
9楼2009-02-24 15:07:20
10楼2009-02-24 15:14:42
