| 查看: 1373 | 回复: 210 | ||
ADTechnology
|
[商家供应]
全球唯一中国现货---尿液样本 m6A 定量检测试剂盒（比色法）（A-P-9015）
|
|
尿液样本 m6A 定量检测试剂盒（比色法） 货号：A-P-9015 MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric) 扫我收藏分享推荐，还有机会拿红包哦！ 背景资料：Nucleobase m6A, a modified form of adenosine converted by adenosine methyltransferases is widespread in different cellular RNAs and also found in DNA. The biological importance of DNA/RNA m6A-methylation as a major epigenetic modification in phenotype and gene expression has been recognized widely. m6A plays crucial roles in regulating DNA replication, DNA damage, RNA splicing, transposition, transcription, and cellular defense. In humans, the m6A modification is probably catalyzed by a methyltransferase complex METTL3/METTL14 and removed by the α-ketoglutarate (α-KG)- and Fe2+-dependent dioxygenases such as FTO, ALKBH5 and TET-like enzymes. It was shown that METTL3 and α-KG /Fe2+-dependent dioxygenases play important roles in many biological processes, ranging from development and metabolism to fertility. Urinary excretion of m6A is an indication of the whole body turnover or the degradation of DNA/RNA, especially tRNA. The urinary m6A level can be changed with a change of the bodies’ turnover of m6A DNA/RNA or alteration of cellular DNA/RNA m6A status. A number of studies have indicated that m6A excreted in urine has the potential to act as a cancer biomarker. For example, an elevated level of urinary m6A was observed in colorectal cancer patients with active disease states。 产品描述：In this ELISA-like inhibitory competitive immunoassay, urine samples and the m6A standard are first incubated with a m6A antibody solution and then transferred to strip wells coated with m6A polynucleotide. The well is washed to remove any unbound reagents after incubation and then a detection antibody is added to generate a signal that can be measured colorimetrically by reading the absorbance in a microplate spectrophotometer. Because m6A in the urine sample inhibits the binding of m6A antibody to m6A coated on the well, higher concentrations of m6A in the urine sample lead to a reduced binding of the antibody to the m6A on the well. Therefore the signal or OD intensity measured from the well will be inversely proportional to the amount of m6A in the urine sample and the amount of m6A in the urine sample can be quantified by a comparison with a predetermined m6A standard。 产品特点： 1·Innovative colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours； 2·96 strip-well microplate format makes the assay flexible: manual or high throughput analysis； 3.Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent； 4.The level of m6A measured in human urine samples using this kit is comparable to that detected by HPLC method； 5.A novel assay principle allows high sensitivity to be achieved. The detection limit can be as low as 0.01 ng/assay well； 6.Low input range of urine for each assay with a volume of 1 to 20 μl and an optimal volume of 5 μl； 7.Optimized antibody and enhancer solutions allow for high specificity to m6A, without cross-reactivity to unmethylated adenosine； 8.Negative control and positive standard are included, which are suitable for quantification of m6A in free form and m6A contained in DNA/RNA fragments from different urine samples。 更多产品链接请猛戳：More  陈相军（背阴+二维码）.jpg  A-P-9015-尿液样本m6A定量检测试剂盒（比色法）.png |
回复此楼» 本帖附件资源列表
-
欢迎监督和反馈：小木虫仅提供交流平台，不对该内容负责。
本内容由用户自主发布，如果其内容涉及到知识产权问题，其责任在于用户本人，如对版权有异议，请联系邮箱：xiaomuchong@tal.com - 附件 1 : A-P-9015-尿液样本m6A_定量检测试剂盒（比色法）.pdf
- 附件 2 : SC-艾德科技-厉害了！！！我的m6A！！！.pdf
- 附件 3 : SC-艾德科技-萌货！奔跑吧！！RNA甲基化时代来了！！！.pdf
2017-10-23 21:59:39, 881.3 K
2017-10-23 22:00:11, 9.66 M
2017-10-23 22:00:15, 2.4 M
» 猜你喜欢
272分材料子求调剂
已经有36人回复
-
275求调剂
已经有8人回复
-
调剂求收留
已经有5人回复
-
一志愿211，化学学硕，310分，本科重点双非，求调剂
已经有20人回复
-
070300化学学硕311分求调剂
已经有19人回复
-
材料与化工调剂
已经有13人回复
-
材料与化工调剂
已经有33人回复
-
复试调剂
已经有7人回复
-
一志愿哈工大 085600 277 12材科基求调剂
已经有17人回复
-
还有化工二轮调剂的学校吗
已经有47人回复
» 抢金币啦！回帖就可以得到:
-
湖北大学 矿物及固废资源化利用环境能源新材料研究团队 材料与化工 调剂招生
+2/440
-
武汉工程大学联合培养招收硕士（食品、环境和化学相关专业）
+1/90
-
广东石油化工学院，材料与化工、能源动力专硕，招收调剂生，线上面试
+2/84
-
河南师范大学材料学院招生调剂生
+2/46
-
招收2026级公共卫生与预防医学专业（学硕）调剂生1名
+1/42
-
B区学生调剂-兰州交通大学材料科学与工程学院
+1/42
-
浙江理工大学蒋仲庆教授课题组招收物理或者材料背景研究生（调剂1名）
+1/27
-
化学、环境工程，上车就走
+1/23
-
一战成硕的绝佳机会，青岛农大草业学院2026年仍有大量调剂名额
+5/20
-
大连大学-贵州省煤炭洁净利用重点实验室联合培养研究生 化学5人+环境工程7人
+1/10
-
湖南理工大学调剂系统已开。 化学、化工、材料化工 调剂指标充足，录取率高，欢迎调剂
+1/8
-
硕士调剂-招新能源电池方向硕士
+1/8
-
【华中科技大学】【管理学院】【吴庆华教授团队】【招收2-3名博士后】
+1/7
-
海南大学徐月山老师招生第二批博士名额1个，2026年9月份入学（高端设备开发方向）
+1/6
-
Top-88悉尼科技大学数据科学/AI 招收2027年入学 校奖 博士生1到2名(国际和本地学生）
+1/5
-
欢迎植物保护、生物等专业调剂至“生物与医药”（0860）硕士研究生
+1/5
-
盐城工学院-工科【招收调剂】！！！
+1/3
-
刘强教授团队招0802机械工程学硕和0855机械工程专硕调剂啦
+1/1
-
江苏大学环境与安全工程学院张祯老师课题组招收环境，化学，机械，电子信息等专业调剂
+1/1
-
招考研调剂生
+1/1
ADTechnology 
捐助贵宾 (著名写手)
   |
2楼2017-10-23 22:02:04
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
|
|
3楼2017-10-23 22:16:53
4楼2017-10-23 22:39:22
5楼2017-10-23 22:56:38
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
|
|
6楼2017-10-23 23:14:04
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
|
|
7楼2017-10-24 07:40:19
8楼2017-10-24 07:55:41
9楼2017-10-24 08:10:19
10楼2017-10-24 08:17:15
