| 查看: 1374 | 回复: 210 | ||
ADTechnology
|
[商家供应]
全球唯一中国现货---尿液样本 m6A 定量检测试剂盒（比色法）（A-P-9015）
|
|
尿液样本 m6A 定量检测试剂盒（比色法） 货号：A-P-9015 MethylFlash Urine N6-methyladenosine (m6A) Quantification Kit (Colorimetric) 扫我收藏分享推荐，还有机会拿红包哦！ 背景资料：Nucleobase m6A, a modified form of adenosine converted by adenosine methyltransferases is widespread in different cellular RNAs and also found in DNA. The biological importance of DNA/RNA m6A-methylation as a major epigenetic modification in phenotype and gene expression has been recognized widely. m6A plays crucial roles in regulating DNA replication, DNA damage, RNA splicing, transposition, transcription, and cellular defense. In humans, the m6A modification is probably catalyzed by a methyltransferase complex METTL3/METTL14 and removed by the α-ketoglutarate (α-KG)- and Fe2+-dependent dioxygenases such as FTO, ALKBH5 and TET-like enzymes. It was shown that METTL3 and α-KG /Fe2+-dependent dioxygenases play important roles in many biological processes, ranging from development and metabolism to fertility. Urinary excretion of m6A is an indication of the whole body turnover or the degradation of DNA/RNA, especially tRNA. The urinary m6A level can be changed with a change of the bodies’ turnover of m6A DNA/RNA or alteration of cellular DNA/RNA m6A status. A number of studies have indicated that m6A excreted in urine has the potential to act as a cancer biomarker. For example, an elevated level of urinary m6A was observed in colorectal cancer patients with active disease states。 产品描述：In this ELISA-like inhibitory competitive immunoassay, urine samples and the m6A standard are first incubated with a m6A antibody solution and then transferred to strip wells coated with m6A polynucleotide. The well is washed to remove any unbound reagents after incubation and then a detection antibody is added to generate a signal that can be measured colorimetrically by reading the absorbance in a microplate spectrophotometer. Because m6A in the urine sample inhibits the binding of m6A antibody to m6A coated on the well, higher concentrations of m6A in the urine sample lead to a reduced binding of the antibody to the m6A on the well. Therefore the signal or OD intensity measured from the well will be inversely proportional to the amount of m6A in the urine sample and the amount of m6A in the urine sample can be quantified by a comparison with a predetermined m6A standard。 产品特点： 1·Innovative colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours； 2·96 strip-well microplate format makes the assay flexible: manual or high throughput analysis； 3.Innovative kit composition enables background signals to be extremely low, which eliminates the need for plate blocking and allows the assay to be simple, accurate, reliable, and consistent； 4.The level of m6A measured in human urine samples using this kit is comparable to that detected by HPLC method； 5.A novel assay principle allows high sensitivity to be achieved. The detection limit can be as low as 0.01 ng/assay well； 6.Low input range of urine for each assay with a volume of 1 to 20 μl and an optimal volume of 5 μl； 7.Optimized antibody and enhancer solutions allow for high specificity to m6A, without cross-reactivity to unmethylated adenosine； 8.Negative control and positive standard are included, which are suitable for quantification of m6A in free form and m6A contained in DNA/RNA fragments from different urine samples。 更多产品链接请猛戳：More  陈相军（背阴+二维码）.jpg  A-P-9015-尿液样本m6A定量检测试剂盒（比色法）.png |
回复此楼» 本帖附件资源列表
-
欢迎监督和反馈：小木虫仅提供交流平台，不对该内容负责。
本内容由用户自主发布，如果其内容涉及到知识产权问题，其责任在于用户本人，如对版权有异议，请联系邮箱：xiaomuchong@tal.com - 附件 1 : A-P-9015-尿液样本m6A_定量检测试剂盒（比色法）.pdf
- 附件 2 : SC-艾德科技-厉害了！！！我的m6A！！！.pdf
- 附件 3 : SC-艾德科技-萌货！奔跑吧！！RNA甲基化时代来了！！！.pdf
2017-10-23 21:59:39, 881.3 K
2017-10-23 22:00:11, 9.66 M
2017-10-23 22:00:15, 2.4 M
» 猜你喜欢
生物学308求调剂（一志愿华东师大）
已经有6人回复
-
调剂求收留
已经有7人回复
-
272分材料子求调剂
已经有36人回复
-
275求调剂
已经有8人回复
-
一志愿211，化学学硕，310分，本科重点双非，求调剂
已经有20人回复
-
070300化学学硕311分求调剂
已经有19人回复
-
材料与化工调剂
已经有13人回复
-
材料与化工调剂
已经有33人回复
-
复试调剂
已经有7人回复
-
一志愿哈工大 085600 277 12材科基求调剂
已经有17人回复
» 抢金币啦！回帖就可以得到:
-
安徽农业大学李亚辉教授招收 化学、农药学、植保类2026级博士研究生
+3/246
-
长江大学化工学院2026年硕士研究生调剂公告（理、工均收）
+1/90
-
供应有机合成一整套设备，有机合成实验室常用产品。
+1/90
-
浙江农林大学竹子研究院招收林业工程专业学硕调剂
+1/89
-
江西师大化学工程学院调剂今晚0点开放！ 缺额100个，速冲！
+1/87
-
延安大学长江学者团队招收材料、化工相关领域的调剂研究生数名
+1/40
-
大连工业大学纺织科学与工程接收硕士调剂
+1/40
-
一志愿前4位代码0703均可报名-河北大学分析化学专业招收调剂考生
+1/24
-
煤炭科学研究总院矿山新材料研究院员工博士后招聘
+1/16
-
南昌航空大学考研调剂招生
+1/13
-
重庆三峡科技大学物理电子学、场波专业接收调剂，考数二可调，系统开至4月10日
+1/12
-
大连大学 化学专业 招收26年硕士研究生调剂
+1/11
-
重庆三峡科技大学物理电子学专业接收调剂，考数二可调，系统开至4月10日，正在报名
+1/11
-
招07及08开头化学、材料、环境、食品、生物与医药等调剂生
+2/6
-
吉林工程技术师范学院 交叉学科研究院（08/07） 招收调剂
+1/5
-
招收化学/材料调剂同学
+1/4
-
上海应用技术大学东方学者特聘教授邓维2026招生调剂研究生
+1/4
-
武汉纺织大学-材料学院-杰青团队招收调剂研究生 尚有少量名额，有意者请尽快联系
+2/4
-
-
+1/2
-
化学、化工、化妆品、材料、环境背景考生调剂
+1/1
ADTechnology 
捐助贵宾 (著名写手)
   |
2楼2017-10-23 22:02:04
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
|
|
3楼2017-10-23 22:16:53
4楼2017-10-23 22:39:22
5楼2017-10-23 22:56:38
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
|
|
6楼2017-10-23 23:14:04
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
|
|
7楼2017-10-24 07:40:19
8楼2017-10-24 07:55:41
9楼2017-10-24 08:10:19
10楼2017-10-24 08:17:15
