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nono2009
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2楼2017-09-23 10:43:00
609718476
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609718476: 金币+2, ★有帮助 2017-09-23 11:02:21
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609718476: 金币+2, ★有帮助 2017-09-23 11:02:21
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Selection of reliable reference genes for gene expression studies in Trichoderma afroharzianum LTR-2 under oxalic acid stress. 作者:Lyu, Yuping; Wu, Xiaoqing; Ren, He; Zhou, Fangyuan; Zhou, Hongzi; Zhang, Xinjian; Yang, Hetong Journal of microbiological methods 卷:141 页:28-31 DOI:10.1016/j.mimet.2017.07.011 出版年:2017-Oct (Epub 2017 Jul 25) 摘要 An appropriate reference gene is required to get reliable results from gene expression analysis by quantitative real-time reverse transcription PCR (qRT-PCR). In order to identify stable and reliable reference genes in Trichoderma afroharzianum under oxalic acid (OA) stress, six commonly used housekeeping genes, i.e., elongation factor 1, ubiquitin, ubiquitin-conjugating enzyme, glyceraldehyde-3-phosphate dehydrogenase, alpha-tubulin, actin, from the effective biocontrol isolate T. afroharzianum strain LTR-2 were tested for their expression during growth in liquid culture amended with OA. Four in silico programs (comparative DeltaCt, NormFinder, geNorm and BestKeeper) were used to evaluate the expression stabilities of six candidate reference genes. The elongation factor 1 gene EF-1 was identified as the most stably expressed reference gene, and was used as the normalizer to quantify the expression level of the oxalate decarboxylase coding gene OXDC in T. afroharzianum strain LTR-2 under OA stress. The result showed that the expression of OXDC was significantly up-regulated as expected. This study provides an effective method to quantify expression changes of target genes in T. afroharzianum under OA stress. Copyright © 2017 Elsevier B.V. All rights reserved. 关键词 关键词列表:Elongation factor; Gene expression; Oxalate decarboxylase; Oxalic acid; Reference gene; Trichoderma afroharzianum 作者信息 地址:College of Life Science, Shandong University of Technology, Zibo 255000, Shandong Province, People's Republic of China; Shandong Provincial Key Laboratory of Applied Microbiology, Ecology Institute, Shandong Academy of Sciences, Jinan 250014, Shandong Province, People's Republic of China. 文献信息 文献类型:Journal Article 语种:English PubMed ID:28754446 NLM 唯一 ID:8306883 创建日期: 29 Jul 2017 修订日期: 04 Sep 2017 电子出版:25 Jul 2017 国家/地区: Netherlands ISSN:1872-8359 其他信息 状态:In-Process |
4楼2017-09-23 10:57:53
609718476
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5楼2017-09-23 11:01:51
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