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COMMENTS TO THE AUTHOR:

Dear Dr. Zhao,

Thank you for resubmitting your revised manuscript. Most of the reviewer comments appear adequately addressed and only a few minor points remain:
76 I believe a little more time needs to be spent on shoot vs. root uptake, perhaps in the discussion if not here, as it is a very important part of the picture
88 rephrase sentence
98 add comma after study
100 specify which variables in "large range of variables",
102 unite paragraphs
122 need to justify why these particular concentrations were chosen
150 rephrase 'leaf and root'
151 ruler
153 use equations editor for the formula
167 move Table A.1 to main text, this information is important; briefly justify parameters in text
206 state whether these transformations have indeed mitigated departure from assumptions
224 N treatment or N concentration...
225 and throughout provide exact p-values (move Table 3 to appendices); note that several p-values would no longer be significant after the correction for multiple tests; please discuss this accordingly.
263 in our experiment, (add comma)
272 need to justify that this list of physiological parameters considered is, in fact, an exhaustive list covering all aspects of plant physiology

We are looking forward to your revised submission,
Sincerely,
Katya Kovalenko

COMMENTS TO THE AUTHOR:

Dear Authors,
thank you for your revision. However, before sending your manuscript in production I still have a couple of small requets.

1. You didn't need to add the authorship in the title. Please remove it.

2. I see that the abstract in the manuscript and the one in the submission form are different. Since the one in the manuscript is longer than allowed (200 words), you probably "cut" the first sentences to go ahead with the submission. However, as a rule, our journal does not accept abstracts longer than 200 words. As a suggestion, you could reduce it as follows (194 words):

The role of nitrogen (N) in the shift from a macrophyte-dominated state to a phytoplankton-dominated one at high N concentrations in shallow lakes is still debated. To elucidate possible toxic and ecological effects of high N on macrophyte growth, we conducted a short-term (40 day) study of a eutrophication-tolerant macrophyte, Vallisneria spinulosa (Hydrocharitaceae), incubated in pots in a mesocosm system subjected to different N concentrations (1, 3, and 5 mg L-1). Plant leaf and root length as well as growth rate decreased significantly with increased N concentrations, but most N- and P-related physiological parameters, including the soluble protein content, nitrate reductase activity, acid phosphatase activity, and tissue N and P contents, did not differ significantly among the N treatments. Only the alkaline phosphatase activity differed, being lower at high nitrogen loading, likely due to P limitation. Epiphyton and phytoplankton biomasses increased significantly with
increasing N loading. Our results including a large number of physiological tests of the macrophytes, therefore, provide supporting evidence that the loss of submerged macrophytes, like V. spinulosa, seen at high N loading in shallow lakes, can be attributed to competition with phytoplankton and epiphyton rather than to toxic effects.

Please resubmit your manuscript without highlighting the changes in "red-text".

Yours sincerely,
Luigi Naselli-Flores
Associate Editor in Chief
Dear Authors,

Thank you for your revised manuscript. I have now the pleasure to accept your paper for publication in Hydrobiologia.

You will be contacted by the manufacturing department in due course. May I ask you to fully collaborate with the production process leading up to publication of your manuscript, i.e. by providing figures of high technical quality if and when necessary and requested, and by returning your proofs within the requested period. Failure to do so might delay the publication of your manuscript, as both scientific and technical quality of Hydrobiologia papers must be of high standard.

Thank you again for submitting your material to Hydrobiologia.
With kindest regards
Luigi Naselli-Flores
Associate Editor in Chief
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