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打哈欠的人金虫 (正式写手)
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[求助]
如何提取小鼠淋巴液？看到有文献需要找乳糜池，请问要怎么找？
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请问各位虫友，应该如何提取小鼠淋巴液？ 看到有文献需要找乳糜池，方法也比较详细：After the mice were killed, the peritoneal cavity was washed with 6 ml of RPMI medium and then its contents were exposed ventrally. About 500 ml of blood was aspirated from the inferior vena cava. Under a stereomicroscope, the cysterna chyli was identified as a small cream-coloured sac located dorsal to the left renal lymph node. A fine borosilicate glass microcapillary pipette was then inserted into the cysterna chyli and lymph fluid was removed and the volume measured with a micropipette. " (doi:10.1038/nature02284) 但是我实际操作起来还是找不到cysterna chyli，翻开小鼠肠道之后下面就是血肉模糊一片了，看不到肾淋巴结。 恳请有经验的站友赐教！ |
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2楼2017-08-17 23:52:46