| 查看: 454 | 回复: 1 | ||
[求助]
30％的丙烯酰胺 已有1人参与
|
|
30％的丙烯酰胺有19:1和29:1，他们是什么意思，我该怎么选择我要用哪个 发自小木虫Android客户端 |
回复此楼» 猜你喜欢
有时候真觉得大城市人没有县城人甚至个体户幸福
已经有3人回复
-
CSC & MSCA 博洛尼亚大学能源材料课题组博士/博士后招生｜MSCA经费充足、排名优
已经有5人回复
-
酰胺脱乙酰基
已经有8人回复
-
有70后还继续奋斗在职场上的吗？
已经有6人回复
-
博士延得我，科研能力直往上蹿
已经有7人回复
-
退学或坚持读
已经有27人回复
-
面上基金申报没有其他的参与者成吗
已经有5人回复
-
遇见不省心的家人很难过
已经有22人回复
zhouxfd
木虫 (正式写手)
- 应助: 158 (高中生)
- 金币: 4721.3
- 散金: 73
- 红花: 22
- 帖子: 697
- 在线: 72.8小时
- 虫号: 1427827
- 注册: 2011-10-05
- 性别: GG
- 专业: 微生物生理与生物化学
【答案】应助回帖
感谢参与，应助指数 +1
|
Control of the pore size of a polyacrylamide gel is accomplished by changing the T and C values. With increasing T, the pore size decreases in a nearly linear relationship. Higher percentage gels (higher T), with smaller pores, are used to separate smaller molecules. The relationship of C to pore size is more complex. Generally, the minimum pore size occurs when C is about 5% (a 19:1 gel). Decreasing C results in a more open pore structure because there are fewer crosslinker molecules. Increasing C beyond 5% also increases the pore size. This appears to be because of nonhomogeneous bundling of strands in the gel. Researchers have settled on C values of 5.0% (19:1 acrylamide/bis) for most forms of denaturing DNA and RNA electrophoresis and 3.3% (29:1) for most native DNA and RNA gels. For SDS-PAGE electrophoresis of proteins, the standard C value that has been adopted is 2.6% (37.5:1). The table below gives recommended acrylamide/bis ratios and gel percentages for different molecular size ranges. Polyacrylamide Gel Solutions Recommended appplications for each for each formulations shown in bold Acrylamide:MBA Ratio Gel % Native DNA/RNA (bp) Denatured DNA/RNA (bp) Protein (kp) 19:1 4 100-1500 70-500 100-200 " 6 60-600 40-400 40-150 " 8 40-500 20-200 20-100 " 10 30-300 15-150 15-70 " 12 20-150 10-100 8-60 29:1 5 200-2000 70-800 >150 " 6 80-800 50-500 50-200 " 8 60-400 30-300 30-125 " 10 50-300 20-200 20-100 " 12 40-200 15-125 10-70 " 20 <40 <40 <30 37.5:1 6 -- -- 60-200 " 8 -- -- 50-150 " 10 -- -- 25-100 " 12 -- -- 15-80 Matrix formulations for various applications. |
2楼2017-07-22 01:22:35