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[求助] 30％的丙烯酰胺已有1人参与

30％的丙烯酰胺有19:1和29:1，他们是什么意思，我该怎么选择我要用哪个

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1楼 2017-07-21 21:26:28

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

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【答案】应助回帖

感谢参与，应助指数 +1

Control of the pore size of a polyacrylamide gel is accomplished by changing the T and C values. With increasing T, the pore size decreases in a nearly linear relationship. Higher percentage gels (higher T), with smaller pores, are used to separate smaller molecules. The relationship of C to pore size is more complex. Generally, the minimum pore size occurs when C is about 5% (a 19:1 gel). Decreasing C results in a more open pore structure because there are fewer crosslinker molecules. Increasing C beyond 5% also increases the pore size. This appears to be because of nonhomogeneous bundling of strands in the gel.

Researchers have settled on C values of 5.0% (19:1 acrylamide/bis) for most forms of denaturing DNA and RNA electrophoresis and 3.3% (29:1) for most native DNA and RNA gels. For SDS-PAGE electrophoresis of proteins, the standard C value that has been adopted is 2.6% (37.5:1). The table below gives recommended acrylamide/bis ratios and gel percentages for different molecular size ranges.

Polyacrylamide Gel Solutions
Recommended appplications for each for each formulations shown in bold
Acrylamide:MBA
Ratio

Gel % Native
DNA/RNA (bp) Denatured
DNA/RNA (bp) Protein (kp)
19:1 4 100-1500 70-500 100-200
" 6 60-600 40-400 40-150
" 8 40-500 20-200 20-100
" 10 30-300 15-150 15-70
" 12 20-150 10-100 8-60
29:1 5 200-2000 70-800 >150
" 6 80-800 50-500 50-200
" 8 60-400 30-300 30-125
" 10 50-300 20-200 20-100
" 12 40-200 15-125 10-70
" 20 <40 <40 <30
37.5:1 6 -- -- 60-200
" 8 -- -- 50-150
" 10 -- -- 25-100
" 12 -- -- 15-80
Matrix formulations for various applications.

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