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Relative tissue distribution and excretion studies of gastrodin and parishin from power and extract of Gastrodiae Rhizoma in rat bx UHPLC-ESI-MS/MS

Zhengmeng Jiang

Guizhou Normal University

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523156717: ½ð±Ò+20 2017-06-09 13:29:01
Relative tissue distribution and excretion studies of gastrodin and parishin from powder and extract of Gastrodiae Rhizoma in rat by UHPLC-ESI-MS/MS.


×÷Õß:Jiang, Zhengmeng; Zheng, Xiuyan; Gong, Xiaojian; Zhao, Chao; Zhou, Xin; Zhao, Yang; Yan, Yanfang




Biomedical chromatography : BMC


DOI:10.1002/bmc.3909

³ö°æÄê:2016-Dec-07 (Epub 2016 Dec 07)


ÕªÒª



New research has indicated that Gastrodiae Rhizome (GR) has potential anti-diabetic and anti-asthmatic effects in mouse models. On the basis of our previous study of the relative bioavailability of gastrodin (GAS) and parishin (PA) from extract and powder of GR, we performed further research on the tissue distribution and excretion of the two analytes. A reliable bioanalytical method for the quantification of GAS and PA in rat tissues and excretion is required. Chromatographic separation was carried out on a gradient mobile phase of acetonitrile-water with 0.1% formic acid. Calibration curves (1/x2 weighted) offered satisfactory linearity (r2 >0.9835) within 100-3000ngmL-1 for GAS and (r2 >0.9862) within 10-1000ngmL-1 for PA. The relative standard deviations of the intra-day and inter-day precision were all <14.98%, whilst the relative errors of the intra-day and inter-day accuracy were all within ¡À14.71%. The matrix effect and recovery values were satisfactory in all of the biological matrices examination. The data of relative differences in tissue distribution and excretion of GAS and PA from powder and extract of GR indicated that higher bioavailabilities for GAS and PA were obtained when a dosage of 4gkg-1 GR powder was used.
Copyright 2016 John Wiley & Sons, Ltd.


¹Ø¼ü´Ê

¹Ø¼ü´ÊÁбí:Gastrodiae Rhizome; UHPLC-ESI-MS/MS; bioavailability; gastrodin and parishin; tissue distribution and excretion studies


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µØÖ·:Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, Guizhou, People's Republic of China.


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ÎÄÏ×ÀàÐÍ:Journal Article

ÓïÖÖ:English

PubMed ID:27925254

NLM Ψһ ID:8610241

´´½¨ÈÕÆÚ:  07 Dec 2016          ÐÞ¶©ÈÕÆÚ:  24 Jan 2017

µç×Ó³ö°æ:07 Dec 2016

¹ú¼Ò/µØÇø: England

ISSN:1099-0801

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4Â¥: Originally posted by tepa2008 at 2017-06-08 13:12:13
Relative tissue distribution and excretion studies of gastrodin and parishin from powder and extract of Gastrodiae Rhizoma in rat by UHPLC-ESI-MS/MS.


×÷Õß:Jiang, Zhengmeng; Zheng, Xiuyan; Gong, ...

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