| 查看: 73 | 回复: 0 | |||
| 当前主题已经存档。 | |||
[交流]
有一小段关于分子的文献不太理解，请求帮助
|
|||
|
原文The overexpression of folA caused a two- fold decrease in folate production compared to that in a con- trol strain or a noninduced strain (Fig. 3). The intracellular folate distribution and the relative amount of polyglutamyl folates remained unchanged. In a similar experiment we stud- ied the effect of the production of antisense RNA encoding dihydrofolate reductase. The complementary sequence of the coding strand of folA was cloned under the control of the nisin promoter nisA in pNZ8048, starting at the 5 end with the complementary sequence of the stop codon of folA and finish- ing at the 3 end with the complementary start codon of the gene. The plasmid generated, pNZ7014, was transformed into L. lactis NZ9000. Under inducing conditions, a small but re- producible increase of approximately 20% in the total folate production was observed compared to that in a control strain (results not shown). To confirm the effect of the transcription of folA antisense RNA, the enzymatic activity of dihydrofolate reductase was determined. Cell extracts of strain NZ9000 har- boring pNZ7014, transcribing antisense RNA of folA, showed a twofold decrease in dihydrofolate reductase activity (Fig. 4). In contrast, cell extracts of L. lactis strains overexpressing folA showed a more-than-1,000-fold increase in dihydrofolate re- ductase activity compared to a control strain |
回复此楼» 猜你喜欢
什么时候开奖？
已经有4人回复
-
咱们一起用铁证分析2026国家社科基金中标与否
已经有27人回复
-
为什么余额宝的年化利率越来越低？主要原因有哪些？
已经有3人回复
-
2027广东省杰青
已经有9人回复
-
朋友圈看到的
已经有5人回复
-
欢迎发来filecode的Mz6后的代码验证其规律
已经有110人回复
-
明天放榜？
已经有5人回复
-
时间戳变了，能看出什么问题？
已经有11人回复
-
重要消息，中午系统在维护
已经有10人回复
-
投稿咨询
已经有6人回复
» 本主题相关商家推荐: (我也要在这里推广)
