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[求助]
光致发光光谱检测H2O2 已有1人参与
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| 各路大神，求光致发光光谱（PL）检测H2O2的方法 |
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SturbonK
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Subsequently, hydrogen peroxide (H2O2) was measured using a (p-hydroxyphenyl) acetic acid (POHPAA) analysis method . Typically, a certain volume of fluorescence reagent (potassium hydrogen phthalate: 8.2 g/L, p-hydroxyphenylacetic acid: 270 mg/L, and type II horseradish peroxidase: 30 mg/L) was preadded into the reaction system. 1.0 mL of sample wgas then withdrawn at an interval time of 2 h and then mixed with 1.0 mL of 1.0 mol/L NaOH for 10 min for measuring the intensity of the fluorescence emission at 409 nm excited at 315 nm. 首先你自己要先做一个标准曲线 |
3楼2017-04-21 14:46:32