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[求助]
20nm的球形纳米银的制备，用来接巯基DNA已有1人参与
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本人想制备20nm纳米银，后续用于接巯基DNA做荧光增强，用2mM硝酸银逐滴加入到两倍体积的3mM的硼氢化钠中，冰浴和磁力搅拌，但是硝酸银滴加不完就会变成黑色聚沉，因为什么原因呢？或者有时候不变黑，滴加完后磁珠上也会有黑色沉淀，制备的银胶会有一些黑色的沉淀 和滴加速度有没有关系，应该用什么速度，都是用注射器手动滴加，或者硼氢化钠和硝酸银的浓度和比例应该怎么合适呢？跪求大神帮助 发自小木虫Android客户端 |
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luojian88
新虫 (正式写手)
- 应助: 15 (小学生)
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- 虫号: 1540049
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- 性别: GG
- 专业: 无机纳米化学
2楼2017-03-29 12:40:51
nanofellow
专家顾问 (著名写手)
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专家经验: +208 - MN-EPI: 6
- 应助: 231 (大学生)
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- 专业: 胶体与界面化学
- 管辖: 微米和纳米
【答案】应助回帖
感谢参与，应助指数 +1
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一般都是用柠檬酸钠稳定的金或银纳米颗粒接巯基DNA，制备单分散性良好的纳米银，可以用我们小组探索的方法，重复性非常好： Wan Y, Guo Z, Jiang X, Fang K, Lu X, Zhang Y, Gu N: Quasi-spherical silver nanoparticles: Aqueous synthesis and size control by the seed-mediated Lee-Meisel method. J Colloid Interface Sci 2013, 394:263-268. Abstract：Silver nanoparticles (AgNPs) are attracting tremendous attention in biomedicine, and their related performances are shape and size-dependent. For biomedical applications, water-soluble AgNPs are necessary. However, aqueous syntheses of AgNPs with controlled shape and size are relatively difficult as the balance between nucleation and growth is hard to regulate. This paper describes a robust method for controllable synthesis of quasi-spherical AgNPs based on the combination of the seed-mediated growth and the Lee–Meisel method by thermal reduction of AgNO3with citrate. In the presented method, citrate-stabilized AgNPs with tunable sizes up to 80 nm were achieved through one-step or stepwise growth process using qualified spherical 4 nm AgNPs as starter seeds. Specially, the two main difficulties (formation of nanorods and secondary nucleation during the growth stage) in the previous studies have been effectively overcome by tailoring the experimental parameters such as the reaction temperature and the seedamount, without extra additives, pH adjustment, and laser treatment. The crucial factors that affect the uniformity of the resulting AgNPs are discussed |
3楼2017-03-29 15:43:59
4楼2017-04-13 11:41:35