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818james

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18409207309: 金币+35, ★有帮助 2017-03-31 07:47:26
18409207309(fjtony163代发): 金币+33, 代发 2017-05-01 11:09:30
fjtony163: 翻译EPI+1, 代发 2017-05-01 11:09:36

The apparent heterogeneous electron
transfer rate constants (ks) of Mb confined to Chit-MWNTs film was evaluated as 5.47 s?1 according to Laviron's
equation. The surface concentration (Γ?) of the electroactive Mb in the Chit-MWNTs film was estimated to be
(4.16±0.35)×10?9 mol cm?2
. Meanwhile, the catalytic ability of Mb toward the reduction of H2O2 was studied.
Its apparent Michaelis–Menten constant for H2O2 was 0.024 mM, showing a good affinity. The linear range forH2O2 determination was from 2.5×10?5 M to 2.0×10?4 M with a detection limit of 1.02×10?6 M (S/N=3).Moreover, the biosensor displays rapid response to H2O2 and good stability and reproducibility.

根据Laviron 公式估算， Chit-MWNTs 膜上的Mb 的表面异相电子转移速率常数为5.47 s?1 。（Chit-MWNTs ）膜里电活性Mb的表面浓度为(4.16±0.35)×10?9 mol cm?2。研究了Mｂ对H2O2 还原的催化能力。Mｂ对H2O2的米氏常数为0.024 mM，表明具有强亲和力；对H2O2 测定的线性范围为2.5×10?5 M to 2.0×10?4 M，测定限制为1.02×10?6 M (S/N=3)。此外，该生物传感器对H2O2 反应迅速，并有高稳定性，高再生性。

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