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第一次投sci，要求大改，第一个审稿人意见不多，第二个审稿人意见很多，有些地方不太懂，麻烦英语好的帮忙看看是什么意思…… 我的论文内容包括植物叶片基因克隆，基因荧光定量表达和枝条电导率，电导率这一块主要是做个佐证。 第二个审稿人意见如下：Main subject taken in the presented manuscript underline the practical importance of selecting frost resistant plants. The molecular mechanism of resistance to frost is not well recognized. Determination and variation of well known genes isolated from the genome of wild apple cultivar, which is a donor of valuable genes, and additionally cultivated in different habitats, will also give the possibility to understand the adaptation mechanisms of this species. The results obtained clearly show the diversity of expression profiles in samples collected from different habitats. Anyway it is not quite clear how the samples were prepared for genotypical/phenotypical analysis. How it was suggested in the title, the manuscript could be focused of characterization of CBF genes for different molecular aspects. The observed results strongly confirm existing data, that genes CBF 1, 2, 3, 4 are activated at the time length of low temperature exposure of seedlings in numerical order. Despite the high quality of the results, they remain inconsistent, and therefore I suggest to describe, or verify the most interesting results in another way, or divide the valuable material within two different publications. 然后还在文章中给了批注：I would suggest a different title, to show the CBF characterization by amino acids sequence analysis and sequence comparison between different species, useful for the phylogenetic tree construction. In this frame I don’t see the necessity to show this data (good material for another publication) 和In my opinion the samples for the expression analysis could be exposed to the temperatures lower than 4oC and the data may be included in the comparison analysis of gene expression profile and phenotypical measurements. In this form, there are two different experiments. 我的文章主要是通过基因表达说明抗寒性强弱，这个审稿人的意见是认为克隆和生理的部分都不需要写进来吗？ 谢谢 发自小木虫IOS客户端 |
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