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艾德科技（北京）有限公司

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表观遗传学（m6A RNA甲基化、RNA甲基化、DNA甲基化、DNA羟甲基、DNA甲酰基、DNA羧基），分子生物学，免疫生物学（一抗、二抗），细胞生物学，实验仪器学（核酸提起仪、超微量分光光度计）等等产品与技术服务

北京市昌平区中关村生命科学园东60米艾德园

联系人: Peter Chen / 邮箱: 1951545998@qq.com / QQ: 1513545070 / 电话: 0313-5935521 / 手机: 18911529660

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[商家供应] Magna m6A RNA甲基化免疫沉淀试剂盒-特定位点检测

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针对特定位点的 m6A RNA甲基化，全球顶级检测方法。具体参考如下：
Magna m6A RNA甲基化免疫沉淀试剂盒-特定位点检测货号：A-17-10499

Magna MeRIP™ m6A Kit- Transcriptome-wide Profiling of N6-Methyladenosine

背景资料：N6-methyladenosine (m6A) represents an abundant RNA modification that is conserved across many different species, ranging from plants, to yeast, to mammals. Similar to the 5-methylcytosine (5-mc) modification of DNA, m6A is a reversible chemical modification of RNA. m6A RNA modifications are an emerging topic in controlling cell fate transitions in mammalian embryonic stem cells. m6A is one of the most prevalent modifications on both mRNAs and noncoding RNAs in eukaryotes with an estimated 12,000 m6A sites in over 7,000 genes. The mark is deposited by a heterodimer of methyltransferase-like 3 and 14 (Mettl3 and Mettl14) and can be removed by the RNA demethylase enzymes FTO and ALKBH5. Obesity risk gene FTO encodes the first identified m6A demethylase. Mutations in FTO have been associated with increased risk for obesity and type II diabetes. Recently, impairment of the status of m6A regulation has lead to prolonged expression of Nanog in ES cells and the inability to exit self-renewal stages toward differentiation。

产品描述：Methylated (m6A) RNA immunoprecipitation (MeRIP) is a method to monitor the status of m6A and map the location of m6A RNA modifications transcriptome-wide. The Magna MeRIP M6A Kit uses the MeRIP method to enable identification and transcriptome-wide profiling of m6A RNA modifications. In the MeRIP assay, RNA is chemically fragmented into 100 nucleotides or smaller fragments followed by magnetic immunoprecipitation with a monoclonal antibody toward m6A. After immunoprecipitation, isolated RNA fragments can be subjected with qRT-PCR or RNA sequencing (MeRIP-seq). The streamlined protocol is easy to process and can produce results with high SN ratios。

产品特点：

1·Complete collection of MeRIP valiidated reagents to streamline the MeRIP method；

2·High signal to noise ratios using high affinity m6A monoclonal antibody and A/G magnetic beads ；

3.Stem cell validated；

4.The Magna MeRIP M6A Kit provides sufficient reagents for 10 MeRIP reactions using 5 μg of mRNA or 5 MeRIP reactions when 300 μg of total RNA is used；

5.Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals。

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。
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附件 1 : A-17-10499-Magna_m6A_RNA甲基化免疫沉淀试剂盒-特定位点分析.pdf

2017-02-09 09:17:27, 1.18 M

附件 2 : A-P-9005-m6A_RNA甲基化定量检测试剂盒（比色法）.pdf

2017-02-09 09:18:02, 1.25 M

附件 3 : SC-艾德科技-绝招在身！走遍天下！掌握表观遗传学前沿产品研究工具！.pdf

2017-02-09 09:18:51, 3.81 M

附件 4 : SC-艾德科技-萌货！奔跑吧！！RNA甲基化时代来了！！！.pdf

2017-02-09 09:18:59, 2.4 M

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