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【有奖交流】积极回复本帖子，参与交流，就有机会分得作者 tjuwyn 的 10 个金币

tjuwyn

新虫 (小有名气)

应助: 0 (幼儿园)
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帖子: 118
在线: 65.6小时
虫号: 1140610
注册: 2010-11-07
专业: 生化分析及生物传感

[交流] 投chem comm，一正一反为什么没有仲裁呢？还可以申诉吗

收到chem comm回复了，一正一反的意见，可是编辑也没有送仲裁，这样还能申诉吗？感觉第二个审稿人有些刁难啊，没有仔细看文章就直接拒了

Thank you for your submission to ChemComm, published by the Royal Society of Chemistry. I sent your manuscript to reviewers and I have now received their reports which are copied below.

After careful evaluation of the manuscript and reviewers’ comments, I regret to inform you that I do not find your manuscript suitable for publication and therefore it has been rejected from ChemComm. Please note that the acceptance rate for ChemComm is ~25% of submitted manuscripts. Criteria for acceptance in ChemComm are that work should be original and significant, and of wide general appeal.

I am sorry not to have better news for you, however, thank you for giving us the opportunity to consider your manuscript. I wish you every success publishing this manuscript elsewhere.

Yours sincerely,
Dr Steven De Feyter
Associate Editor, ChemComm

************
REVIEWER REPORT(S):
Referee: 1

Comments to the Author
Wang et al. designed a novel method for multiplexed miRNA detection by coupling DSN and TDT. It is interesting and the data is sufficient. However, some issues need to be addressed before the manuscript is published in Chemical Communications. The detailed problems are as follow.
1. The operation of the method require several performing steps, such as the DNA-assisted digestion for 2 h at 50°C，TDT-induced labeling for 2 h at 37°C and SA–PE binding reaction for 30 min at 37°C. Therefore, the experiment is time-consuming and complex. The advantages of the method should be provided.
2. Let family includes many mutation types, such as let-7b and let-7d with two base mutation and let-7e with single base mutation. Can the method for let-7a detection discriminate against them?
3. In Page 5 of 17, “Figure 2A” should be added to the content “……with the logarithm of the concentration of let-7a in the range of 1 amol to 10 fmol ……”
4. Please provide the background information about detection of miRNA, and cite more references about detection of miRNA by DSN enzyme

Referee: 2

Comments to the Author
In this work, the authors reported an in vitro assay for multiplexing detection of microRNAs based on xMAP platform. This platform could achieve outstanding performance in developing detection methods with high throughput. However, this reported method was one of the in vitro assays anyway, which have been extensively reported these years. Meanwhile, the dual enzymatic signal amplification strategy in this work was quite common. In my opinion, if this work could display the distribution of microRNAs in vivo, it will be much more interesting. Therefore, I don’t suggest this manuscript be published by Chemical Communications. The comments are as follows:
1. The signal amplification strategy is quite common as it is the direct cascade of two widely used nucleic acid signal amplification strategies. [J. Am. Chem. Soc., 2012, 134, 5064–5067; Chem. Commun., 2013, 49, 7243-7245; Anal. Chem., 2014, 86, 1361–1365; Anal. Chem., 2014, 86, 1361–1365; Anal. Chem., 2014, 86, 11913–11918; ACS Appl. Mater. Interfaces, 2015, 7, 24046–24052]
2. The authors’ group have reported the multiplexing detection of miRNAs based on xMAP platform early in 2014 [Anal. Chem., 2014, 86, 10148–10156]. Comparing to the reported work, this work haven’t present any innovation in the usage of xMAP technology.
3. The prototype of some abbreviations should be explained in the main manuscript, such as SA and PE.
4. It will be more interesting if this method could distinguish different living cancer cells.

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