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½áÌâÓ¢ÎÄÕªÒª        This work was carried out systematically to investigate the affects of chemical constituents, afficacy,toxicity and inorganic elements of RADIX PAEONIAE ALBA and RADIX ANGELICAE DAHURICAE by sulfur-fumigated process. The results showed that the main chemical constituents changed in quality and quantity and new chemical constituents appeared in RADIX PAEONIAE ALBA and RADIX ANGELICAE DAHURICAE before and after sulfur-fumigated process through HPLC,HPLC-MS qualitative and HPLC quantitative analyses. The results suggested that sulfur-fumigated process could result in the residues of sulfur,and some heavy metals, such as As,Hg,Pb, and meantime the content changes of some trace elements by using ICP-AES analytical method to determine the harmful heavy metals, sulfur and the main trace elements in RADIX PAEONIAE ALBA and RADIX ANGELICAE DAHURICAE before and after sulfur-fumigated process. A rapid detection method coupled with IR spectroscopy for distinguishing the sulfur-fumigated medicinal materials was established. The results showed that the afficacies in sulfur-fumigated groups were decreased compare to that in non sulfur-fumigated groups in anti-inflammatory experiment by anti-inflammatory and analgesic investigations to the sulfur-fumigated affects in RADIX PAEONIAE ALBA and RADIX ANGELICAE DAHURICAE. Through the experimental investigations of acute and chronic toxicities to sulfur-fumigated medicinal materials, the results of acute toxicity showed that the toxicity of sulfur-fumigated RADIX PAEONIAE ALBA was stronger than that of non sulfur-fumigated one, and the toxicity of non sulfur-fumigated RADIX ANGELICAE DAHURICAE was stronger than that of sulfur-fumigated one. The blood biochemical indexes of chronic toxicity showed that the administrations of sulfur-fumigated medicinal materials had great affects to creatinine, urea nitrogen, total Bilirubin and all kinds of inorganic salt ions in rats, suggested that sulfur-fumigated medicinal materials might affect excretion ,metabolism and the functions of liver and kidney. The results of histopathology of chronic toxicity showed that the toxicity to rats' kidneys with sulfur-fumigated RADIX PAEONIAE ALBA might be reversible, and the toxicity to rats' livers and kidneys with sulfur-fumigated RADIX ANGELICAE DAHURICAE might be irreversible under given dosages.
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