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虫号: 3457579
注册: 2014-10-06
专业: 微生物药物

[求助] 请问检测siRNA脱靶效应的实验图怎么看呀？

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To determine whether one or more of miR-1298 targets contributed to the synthetic lethality of miR-1298 in KRAS MUT cell lines, we knocked down FAK, LAMB3 using SMARTpool siRNAs. Downregulation of both FAK and LAMB3 impaired the viability of KRAS MUT cells.To rule out possible off-target effects of the LAMB3 and FAK siRNAs, we tested four siRNAs against each gene, and found that three of the four siRNAs effectively killed KRAS MUT cells（如下图）.

图的说明：Viability of the indicated panel of KRAS WT (blue) and KRAS MUT (red) NSCLC lines 5 days after transfection with four individual siRNAs against LAMB3 (A) and FAK (B). Each cell line was tested in duplicate. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.

我想问下这样的结果是说明LAMB3和FAK的siRNA脱靶效应较小吗？麻烦解释详细一点，我比较笨..

数据图

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