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[求助]
变异链球菌Ingbritt株 已有2人参与
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变异链球菌Ingbritt株的公认编号有没有呢？比如ATCC、CCTCC。它的菌种来源是哪里呢？临床分离？如果是国际标准菌株，为什么没有标准编号呢？它跟UA159有什么关系？准备做蛋白组学，但在uniprot 数据库里查不到这个变异链球菌的这种菌株，也就找不到标准菌株蛋白数据库，只查的到UA159的。 @天使托 发自小木虫IOS客户端 |
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2楼2016-11-15 17:50:40
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诗意花香: 金币+5 2016-11-17 10:28:41
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诗意花香: 金币+5 2016-11-17 10:28:41
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这是伯杰氏手册里介绍变异链球菌那节~ 32. Streptococcus mutans Clarke 1924, 144AL mu′tans. L. part. adj. mutans changing. Cells are coccoid, approximately 0.5–0.75 μm in diameter,occurring in pairs or as short- to medium-length chains without capsules. In acid conditions in broth and on some solid media, Streptococcus mutans may form short rods (1.5–3.0 μm in length) and rod-shaped morphology may be evident on primary isolation from oral specimens.Growth on blood agar after incubation anaerobically for 2 d produces colonies that are white or gray, circular or irregular, 0.5–1.0 mm in diameter, sometimes rather hard and tending to adhere to the surface of the agar and slightly pitting into the agar surface. Hemolytic reaction on blood agar is usually α-hemolytic or non-hemolytic with very occasionally strains giving β-hemolysis. Growth on sucrose-containing agar typically produces rough, heaped colonies, about 1 mm in diameter with soluble extracellular polysaccharide visible as beads, droplets, or puddles of liquid on or surrounding the colonies. Some strains may form smooth or mucoid colonies (Edwardsson, 1968,1970). Facultatively anaerobic; while most strains grow in air growth is optimum at 37°C under anaerobic condition with some strains CO2-dependent. A few strains have been reported to grow at 45°C, but no growth occurs at 10°C. Acid is produced from arbutin, d-cellobiose, esculin, galactose, glucose, inulin, lactose, maltose, mannitol, mannose, N-acetylglucosamine, salicin, sorbitol, sucrose, trehalose, and d-raffinose. Melibiose and amygdalin fermentation are variable characteristics of Streptococcus mutans with strainsthat cannot ferment melibiose being less frequently able to produce acid from raffinose or amygdalin. Acid is notproduced from adonitol, arabinose, cyclodextrin, dulcitol,erythritol, glycerol, glycogen, gluconate, inositol, methyl d-glucoside, methyl d-mannoside, methyl d-xyloside, melezitose, rhamnose, ribose, sorbose, starch, or xylose.Strains hydrolyze esculin but not arginine. Starch is hydrolyzed by the majority of strains but hippurate and urea are not. Strains produce leucine aminopeptidase, valine aminopeptidase, chymotrypsin, phosphoamidase, α- and β-glucosidase, α-galactosidase, and β-lactosidase. Strains do not produce alkaline phosphatase, β-glucosaminidase, glycyl tryptophan arylamidase, N-acetyl-β-d-galactosaminidase, N-acetyl-β-d-glucosaminidase, β-d-glucuronidase, α-lfucosidase, β-d-fucosidase, β-mannosidase, β-maltosidase, α-arabinosidase, pyrrolidonyl arylamidase, urease, hyaluronidase, orneuraminidase (sialidase). α-maltosidase is a variable characteristic of this species. Melibiose nonfermenting strains do not produce α-galactosidase or α-glucosidase. Test results for β-d-galactosidase production have been shown to vary depending on the substrate derivative employed (Kilian et al., 1989b). Acetoin is produced by the majority of strains. Hydrogen peroxide is not produced.Strains are non-groupable with Lancefield grouping antisera. Strains contain the cell-wall polysaccharide-typing antigens c, e, or f, according to the serological scheme of Bratthall (1970) and Perch et al. (1974). Extracellular polysaccharide (dextran) is produced from sucrose. The majority of strains are resistant to bacitracin. Strains are isolated from the oral cavity where the primary habitat is the tooth surface (dental plaque) and colonization is favored by high levels of dietary sucrose. Strains have been isolated from feces. 16S rRNA gene sequence analysis places this species within the Mutans species group. DNA G+C content (mol%): 36–38 (Tm and buoyant density). Type strain: ATCC 25175, CCUG 6519, CCUG 11877,CCUG 17824, CIP 103220, DSM 20523, HAMBI 1519,JCM 5705, LMG 14558, NBRC 13955, NCTC 10449. GenBank accession number (16S rRNA gene): AJ243965,AY188348, X58303. Additional comments: The complete genome sequencehas been determined for strain UA159 (GenBank accession no.AE0141333) composed of 2,030,936 bp with a mean G+C content of 36.82 mol%. The genome contains 1963 open reading frames (ORFs) of which 63% were assigned putative functions at the time of publication(Ajdic et al., 2002). Analysis has shown that almost 15% of the genome is accounted for by non-oxidative pathways for carbohydrate metabolism and associated transport systems and reinforces the suggestion that Streptococcus mutans is able to metabolize a wider range of carbohydrates than any other Gram-positive bacterium sequenced to date. A recent publication has described an additional serotype (k) within this species. Serotype k strains were isolated from the dental plaque of children and were less susceptible to phagocytosis than other serotypes (Nakano et al.,2004). 在里面没看到关于变异链球菌Ingbritt株的描述~而且我在NCBI上查到了Ingbritt相应的信息~但是菌种来源直接写的是变异链球菌，没有具体编号。我发现中文文献上有这个~说是变异链球菌血清C型~但是确实没有查到相关信息。PS：NCBI上变异链球菌血清C型显示的是Streptococcus mutans serotype C，而这个是Streptococcus mutans MT6R。还有，文献说变异链球菌Ingbritt株就是strA基因缺失了11bp核苷酸序列的突变株而已~希望能帮到你~ |
3楼2016-11-15 18:46:33
4楼2016-11-16 09:00:24
jql81070305
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5楼2016-11-16 20:23:40