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[求助] Genebank提交序列遇到问题？该如何解决？

Some or all of the protein coding sequences contain reading frame shifts?
(insertions/deletions based on BLAST similarity search results and/or an?
alignment), and/or have translations that show little or no similarity?
to other proteins in the database.?

This generally indicates problems such as:?

?- errors in the nucleotide sequence?
?- sequences have not been trimmed of low quality?
?- problems with feature annotation which may be due to:?
? ? ?translation in the wrong reading frame or on the wrong strand?
? ? ?translation does not have the correct exon spans
? ? ?translation includes noncoding sequence, such as introns or UTR's?
? ? ? ??
If the sequences and/or annotation are incorrect, please resubmit?
the complete file(s) including the revised sequences. For additional?
assistance, please see:?

https://ncbiinsights.ncbi.nlm.ni ... coding-region-cds/?

If you are unable to determine the correct sequence and/or annotation?
for your sequence(s), they may be trimmed at the ends to resolve sequence?
issues or the following comment may be added to one or more of your?
record(s):?

COMMENT ?GenBank staff is unable to verify sequence and/or annotation?
? ? ? ? provided by the submitter.?
? ? ??
In addition, the record(s) will indicate that the data are unverified and?
your sequence(s) will not be included in NCBI BLAST databases.?

Please either:?

? ? ?[1] resubmit with correct sequence(s) and/or annotation?
? ? ?or?
? ? ?[2] confirm that your sequence(s) should be trimmed or?
? ? ? ? ? ?unverified; note we will only be able to trim if it?
? ? ? ? ?involves a few of the records?

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