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electrchem

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[交流] 波兰博士招生（转发）

Chemosensors for selective determination of autism biomarkers
波兰导师：Wlodzimierz Kutner, Piyush Sindhu Sharma
法国合作导师：Mathieu Etienne, LCPME, CNRS（本人同事）

Several recent studies proposed determining levels of the gamma aminobutyric acid (GABA), melatonin & oxytocine nonapeptide biocompounds in body fluids for early autism spectrum disorder (ASD) diagnoses in children. That way, these biocompounds serve as the ASD biomarkers. Melatonin is an endogenous neurohormone predominantly produced in the pineal gland. It is synthesized from L-tryptophan through several metabolic intermediates, most notably serotonin. Physiological levels of melatonin and its derivates are commonly below average in the ASD patients and correlate well with autistic behaviour. Very similarly, recent reports suggested that social impairments found in these patients are associated with changes in the GABA and oxytocin levels in body fluids. In addition to these biomolecular compounds, the recent research confirms the presence of some microorganisms, such as Clostridium bolteae, in stool samples of autism patients. Therefore, detection of these microorganisms will help in early diagnosis of autism. The task of imprinting of whole bacteria is challenging. Therefore, first, we will use model bacteria for developing a procedure of this imprinting. Inspiration for this PhD topic comes from the publications of P. S. Sharma group (Analytica Chimica Acta, 844 (2014) 61-69), and M.Etienne group (Electrochemically assisted bacteria encapsulation in thin hybrid sol–gel films, Journal of Materials Chemistry B, 1 (2013) 1052-1059).

The goal: The long-term perspective is to devise and fabricate robust, efficient, analytically validated molecularly imprinted polymer (MIP) devices as well as to develop procedures for selective quantification of biomarkers in body fluids for early autism diagnosis.

Our approach: To develop chemosensors for above-mentioned biomarkers, the well know technique of molecular imprinting will be applied. The resulting imprinted polymers, in a form of recognition units, will be integrated with different transducers for chemosensor preparation. We will explore several new transducers including surface plasmon resonance (SPR) chips & chemFETs. In the SPR transduction, adsorption or interaction of an analyte with the MIP deposited on the gold chip surface will change the refractive index. In chemFETs, the transduction mechanism will involve chemical modulation of the work function of the gate MIP material by the analyte.

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