Znn3bq.jpeg
ÉÇÍ·´óѧº£Ñó¿ÆÑ§½ÓÊܵ÷¼Á
²é¿´: 280  |  »Ø¸´: 1
µ±Ç°Ö÷ÌâÒѾ­´æµµ¡£

pearshunshun

½ð³æ (СÓÐÃûÆø)

[½»Á÷] 20½ð±Ò½±Àø£¡Çë½ÌÄÉÃ×½ð±ê¼ÇÄýѪøÊÊÅäÌåµÄʵÑéϸ½ÚÎÊÌ⣿£¨ÓÐЧÆÚµ½08Äê12ÔÂ5ÈÕ£©£©

Sample Text  Îȶ¨ÄÉÃ×½ðµÄÊÊÅäÌåµÄÓÃÁ¿ÎÒÒѾ­È·¶¨Á˵ģ¬È»ºóÎÒÓÃ200uLµÄ0.01%ÄÉÃ×½ðÓëÒ»¶¨Á¿µÄ29¸ö¼î»ùµÄÄýѪøÊÊÅäÌå»ìºÏÒÔºó£¬ÔÚ25¶ÈÎÂÓýÁË30·ÖÖÓ£¬ÔÙ¼ÓÈëTris-HCl »º³åÈÜÒººÍNaCl£¬È»ºó¼ÓÈëÒ»¶¨Á¿µÄÄýѪøÓëÖ®·´Ó¦£¬ÔÙ¼ÓÈëNaCl¡£µ«ÊÇÈ´·¢ÏÖ¼ÓÈëÄýѪøµÄÌåϵºÍûÓмÓÈëÄýѪøµÄÌåϵ¶¼Ã»ÓÐÄÉÃ×½ð¾Û¾ÛµÄÏÖÏó£¬Ò²¾ÍÊÇûÓбä³É×ÏÉ«¡£¼ÌÐøÔö´ó¼ÓÈëµÄÂÈ»¯ÄÆÅ¨¶ÈºÍÄýѪøŨ¶È£¨×î¸ß´ïµ½10U/mL£©¶¼Ã»Óп´µ½¾Û¼¯ÏÖÏ󣬷´Ó¦¾ùÔÚ25¶ÈÏ·´Ó¦µÄ¡£

¶ÔÓÚÕâ¸öÏÖÏó£¬ÎÒÓм¸¸öÒÉÎÊ£º
1¡¢²»ÊÇÒòΪÊÊÅäÌåûÓкͽðÄÉÃ×½áºÏºÃ£¬µ¼ÖÂÄýѪøµÄ¼ÓÈëûÓÐʹµÃÄÉÃ×½ð¾Û¼¯£¬ÄÇôÊÊÅäÌåºÍÄÉÃ×½ð½áºÏµÄʱ¼äÓ¦¸ÃÔõô¿ØÖÆ£¿
2¡¢ÊÇ·ñÒòΪÄýѪøÓëÊÊÅäÌå½áºÏ·´Ó¦µÄʱ¼äºÍÌõ¼þ²»¶Ô£¬µ¼ÖÂÄýѪø¸ù±¾Ã»ÓкÍÊÊÅäÌå½áºÏ£¬ÄÇôÕâ¸öʱ¼äºÍÌõ¼þÊÇʲô£¿

3¡¢»º³åÒººÍµç½âÖÊÂÈ»¯ÄÆ¡¢ÂÈ»¯¼ØÊÇÓ¦¸ÃÓëÊÊÅäÌå»ìºÏºó¼ÓÈëÄÉÃ׽𣻻¹ÊÇÓ¦¸ÃÔÚÄÉÃ×½ðºÍÊÊÅäÌå½áºÏÒÔºóÔÙ¼Ó½øÈ¥»º³åÈÜÒººÍµç½âÖÊÂÈ»¯ÄƺÍÂÈ»¯¼Ø£¿

ÏëÇë½ÌÒ»ÏÂÓÐ×ö¹ýÕâ·½ÃæÊµÑéµÄÅóÓÑÖ¸µãһϣ¿


ÎÒÊDzο¼ÁËÏÂÃæÒ»ÆªÎÄÕÂÒÔ¼°ÕâÆªÎÄÕµIJ¹³ä²ÄÁÏÀ´×öÕâ¸öʵÑéµÄ¡£

[1]Simple and Sensitive Aptamer-based Colorimetric Sensing of Protein
Using Unmodified Gold Nanoparticle Probes[J].Hui Wei, Bingling Li, Jing Li, Erkang Wang and Shaojun Dong.Chem. Commun., 2007, 3735¨C3737 .£¨ÎÄÏ×±£´æÔÚÍøÂçÏÂÔØµØÖ·ÖУ¬¿ÉÏÂÔØ£©

ÆäÖеÄÖ÷ҪʵÑé²½ÖèÈçÏ£º

Preparation of Gold Nanoparticles (AuNPs). 13 nm AuNPs were prepared according to a literature method. Briefly, a sodium citrate solution (0.1 M, 1.94 mL) was rapidly added to a boiled HAuCl4 solution (50 mL H2O, 0.167 mL 10% HAuCl4) under vigorous stirring. The mixed solution was boiled for 10 min and further stirred for 15 min. The resulting wine-red solution was cooled to room temperature and filtered, which was stored in the 4 ¡ãC refrigerator before use.

Colorimetric Detection of Thrombin. A typical colorimetric analysis was realized as following procedure £ºfirst, 200 ¦ÌL 13 nm AuNPs was mixed with 30 ¦ÌL 4.59 ¦ÌM 29-mer thrombin binding aptamer (or control random oligonucleotide) in 20 mM Tris-HCl buffer containing 140 mM NaCl, 5 mM KCl (pH 7.5). Second, 30 ¦ÌL thrombin with appropriate concentration (or BSA) in water was added to the AuNPs/aptamer solution. The solutions were allowed react for 5 min at room temperature and then 100 ¦ÌL 0.5 M NaCl was added to produce color change. Time-dependent UVvisible spectra of the mixing AuNPs/aptamer/thrombin solution in the presence of NaCl were then measured with a Cary 500 Scan UV-Vis-NIR Spectrophotometer.Sample Text
http://www.namipan.com/d/%e5%87%9d%e8%a1%80%e9%85%b6%e9%80%82%e9%85%8d%e4%bd%93Simple%20and%20sensitive%20aptamer-based%20colorimetric%20sensing%20of%20protein%20using%20unmodified%20gold%20nanoparticle%20probes.pdf/6a5c9ffcfc354623a20809ffc4b8548747c503ec66280400

[ Last edited by pearshunshun on 2008-12-2 at 18:30 ]

» ²ÂÄãϲ»¶

Ï£ÍûÓë³æÓÑÃǶà¶à½»Á÷,¹²Í¬½ø²½ºÇ!
ÒÑÔÄ   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

chjw2010

Ìú¸Ëľ³æ (ÖøÃûдÊÖ)

ÏîÄ¿¾­Àí

¸øÄ㶥һϰÉ,˳±ã׬¸ö±Ò±Ò
еÄÒ»Äê!
2Â¥2008-11-30 12:01:30
ÒÑÔÄ   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ pearshunshun µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] ҩѧ305Çóµ÷¼Á +6 Â꿨°Í¿¨boom 2026-04-11 6/300 2026-04-14 19:48 by zhouxiaoyu
[¿¼ÑÐ] Çóµ÷¼Á +9 СÄô°®Ñ§Ï° 2026-04-11 13/650 2026-04-14 19:18 by Art1977
[¿¼ÑÐ] 0854µ÷¼Á +11 ³¤¹­°Á 2026-04-12 14/700 2026-04-14 16:07 by ÄæË®³Ë·ç
[¿¼ÑÐ] 297£¬¹¤¿Æµ÷¼Á? +6 ºÓÄÏũҵ´óѧ-ÄÜ 2026-04-14 6/300 2026-04-14 16:04 by Art1977
[¿¼ÑÐ] Ò»Ö¾Ô¸085502£¬267·ÖÇóµ÷¼Á +19 ÔÙæҲҪ³Ô·¹°¡ 2026-04-08 20/1000 2026-04-14 16:03 by zs92450
[¿¼ÑÐ] ҩѧ305Çóµ÷¼Á +10 Â꿨°Í¿¨boom 2026-04-10 10/500 2026-04-14 15:55 by zs92450
[¿¼ÑÐ] ²ÄÁÏ085601µ÷¼Á +32 ºÎÈó²É123 2026-04-10 34/1700 2026-04-14 08:47 by ľľmumu¡«
[¿¼ÑÐ] ¿¼ÑÐÓ¢Ò»ÊýÒ»338·Ö +9 ³¤½­´óѧ¶«Ð£Çø 2026-04-13 10/500 2026-04-14 00:41 by Íõ¬Bè±
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤300Çóµ÷¼Á +39 Ф¿ªÎÄ 2026-04-09 43/2150 2026-04-12 01:30 by Çï¶¹²ËÑ¿
[¿¼ÑÐ] 086003µ÷¼ÁÇóÖú +21 ËÕß®Íò 2026-04-09 22/1100 2026-04-11 20:25 by dongdian1
[¿¼ÑÐ] 337Çóµ÷¼Á +4 ÑÐs. 2026-04-10 4/200 2026-04-11 08:57 by zhq0425
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤µ÷¼Á +12 ·ñ¼«Ì©À´2026 2026-04-10 13/650 2026-04-11 00:28 by wangjihu
[¿¼ÑÐ] 0858Çóµ÷¼Á 5+5 Gky09300550£¬ 2026-04-10 8/400 2026-04-10 19:13 by chemisry
[¿¼ÑÐ] ±¾9 Ò»Ö¾Ô¸Î÷¹¤´ó085601 324Çóµ÷¼Á +5 wysyjs25 2026-04-10 5/250 2026-04-10 16:57 by luoyongfeng
[¿¼ÑÐ] Ò»Ö¾Ô¸»¦9£¬326ÉúÎïѧÇóÏà¹Ø×¨Òµµ÷¼Á +4 Áõīī 2026-04-09 4/200 2026-04-10 12:07 by pengliang8036
[¿¼ÑÐ] µ÷¼ÁÉêÇë086000Ò»Ö¾Ô¸Î÷±±Å©ÁֿƼ¼´óѧÉúÎïÓëÒ½Ò©320·Ö-±¾¿ÆÆë³¹¤Òµ´óѧ +3 ÃÀÃÀŮʿ 2026-04-09 3/150 2026-04-10 10:31 by liuhuiying09
[¿¼ÑÐ] ±¾¿ÆÎ÷¹¤´ó 0856 324Çóµ÷¼Á +10 wysyjs25 2026-04-09 11/550 2026-04-10 08:37 by 5268321
[¿¼ÑÐ] ¿¼Ñе÷¼Á-²ÄÁÏÀà-284 +28 Ïë»»ÊÖ»ú²»Ïë½âÊ 2026-04-08 28/1400 2026-04-09 20:08 by µ¹Êý321?
[¿¼ÑÐ] 085501»úеӢ¶þ77×Ü·Ö294Çóµ÷¼Á£¬½ÓÊÜ¿çרҵѧϰ +6 ÊØ·¨¹«ÃñØÁ¼Í 2026-04-08 6/300 2026-04-09 15:55 by wp06
[¿¼ÑÐ] 086004 Çóµ÷¼Á 309 +7 Yin DY 2026-04-08 7/350 2026-04-09 13:59 by Delta2012
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û