| 查看: 288 | 回复: 0 | ||
[求助]
大侠们，帮女子一把，谢谢
|
各位过路高手，麻烦高抬贵手，帮帮忙解释下面这段的具体操作步骤，看不懂 Metabolic labeling of cellular DNA using synthetic nucleosides. Cells were seeded in 100 mm round cell culture dishes (13 mL) containing glass coverslips (VWR, thickness 1.5, diameter 13 mm) at 100’000 - 300’000 cells per mL and incubated overnight to ensure an even distribution of cells. The coverslips were placed in 24- well plates containing fresh media solutions with variable concentrations of nucleosides (diluted from appropriate stock solutions in DMSO). After incubating for various times, the cells were fixed in paraformaldehyde (3.7%) for 15 min at room temperature, quenched with PBS containing 50 mM glycine and 50 mM NH4Cl for 5 min, and washed twice with PBS. Samples were then stained via invDA or CuAAC. @hc-material |
回复此楼» 猜你喜欢
售SCI一区T0P文章，我:8.O.55.1.O.5.4,科目全,可+急
已经有6人回复
-
售SCI文章，我:8O5.5.1.O.54,科目齐全,可+急
已经有6人回复
-
【长期招聘】复旦大学 AI 制药实验室 博士后招聘
已经有11人回复
-
售SCI一区T0P文章，我:8.O.55.1.O.5.4,科目全,可+急
已经有7人回复
-
2027博士申请-临床检验诊断学、病原生物学、微生物方向
已经有5人回复
-
各位基础老师们，看完中央《从严治党》的决定后，对学术界更加有信心了吧
已经有4人回复
-
申博自荐 2篇一区TOP+1篇EI+2公开发明专利 2027博士申请
已经有3人回复