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[求助]
大侠们，帮女子一把，谢谢
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各位过路高手，麻烦高抬贵手，帮帮忙解释下面这段的具体操作步骤，看不懂 Metabolic labeling of cellular DNA using synthetic nucleosides. Cells were seeded in 100 mm round cell culture dishes (13 mL) containing glass coverslips (VWR, thickness 1.5, diameter 13 mm) at 100’000 - 300’000 cells per mL and incubated overnight to ensure an even distribution of cells. The coverslips were placed in 24- well plates containing fresh media solutions with variable concentrations of nucleosides (diluted from appropriate stock solutions in DMSO). After incubating for various times, the cells were fixed in paraformaldehyde (3.7%) for 15 min at room temperature, quenched with PBS containing 50 mM glycine and 50 mM NH4Cl for 5 min, and washed twice with PBS. Samples were then stained via invDA or CuAAC. @hc-material |
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