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brightfuture01木虫之王 (文坛精英)
我爱打老虎
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[交流]
帮忙啊,大家谁知道Q-Sepharose 是那种胶?
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Purification of DNA Q-Sepharose (Sigma) was washed and equilibrated with 10 mM potassium phosphate buffer (pH 7.2). Finally, approximately 500 ll of solid washed beads were suspended in 1.5 ml of 10 mM potassium phosphate buffer. After mixing thoroughly the aliquots of 300 ll of Q-Sepharose in buffer were transferred in 5 eppendorf tubes and centrifuged for 1 min to separate the overlaying buffer. Different concentrations of chromosomal DNA preparation (100–500 lg) in 1 ml TE buffer were added in the eppendorf tubes containing Q-Sepharose beads and mixed properly by inverting the tubes up and down slowly for 15 min and were kept at room temperature for 5 min. The brown color in the chromosomal preparation bound instantly to the Q-Sepharose. The preparations were centrifuged at 1000 · g for 1 min. Supernatant containing DNA was saved for spectrophotometric analysis as well as for restriction digestion analysis of metagenomic DNA with Sau3A1as above. 但是我翻看sigma 的产品目录发现有N种他所说的(见sigma 产品手册1777页). sepharose LH-20, 2B,4B,6B,CL-2B,4B,6B.大家有知道作者所指的是哪个么?小弟 将会非常感激. [ Last edited by brightfuture01 on 2008-11-26 at 20:58 ] |
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