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2.3. FabricationofAuNPs/OPD
AuNPsweresynthesizedusingamodified citratereduction
method (Lee etal.,1982). Briefly,50mLof0.05wt%HAuCl4 solution
washeatedundervigorousstirring.Afterheatingtobeboil-
ing, 5mLsodiumcitratesolution(1.00wt%)wasaddedunder
continuous stirring.Themixedsolutionwasthenkeptboilingfor
about 10minbeforecoolingtoatmospherictemperature.The
obtained AuNPswerecentrifugedandresuspendedin50mLof
ultrapurewater,thenstoredat4 ¡ãC forthefollowingusage.An
OPD solutionwhichwaspreparedfreshlywasaddeddropwise
intotheobtainedAuNPscolloidstomodifythesurfaceofAuNPs
with alayerofOPD.Afterincubationfor30min,centrifugation
and resuspensioninphosphatebufferedsaline(PBS,pH¼7.4)
wereconductedthreetimestoremovetheexcessOPDinthe
mixture.
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