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1. Synthesis of 4-thio-5-(2¡±-thienyl)uridine and cytotoxicity activity against colon cancer cells in vitro 2. Bacillus vini sp. nov. isolated from alcohol fermentation pit mud 3.Complete genome sequence of strain Lentibacillus amyloliquefaciens LAM0015T isolated from saline sediment 4.Highly efficient production of optically pure l-lactic acid from corn stover hydrolysate by thermophilic Bacillus coagulans |
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Bacillus vini sp nov isolated from alcohol fermentation pit mud ×÷Õß:Ma, KD (Ma, Kedong)[ 1,2 ] ; Chen, XR (Chen, Xiaorong)[ 3 ] ; Guo, X (Guo, Xiang)[ 4 ] ; Wang, YW (Wang, Yanwei)[ 4 ] ; Wang, HM (Wang, Huimin)[ 2 ] ; Zhou, S (Zhou, Shan)[ 2 ] ; Song, JL (Song, Jinlong)[ 5 ] ; Kong, DL (Kong, Delong)[ 2 ] ; Zhu, J (Zhu, Jie)[ 2 ] ; Dong, WW (Dong, Weiwei)[ 2 ] ¸ü¶àÄÚÈÝ ARCHIVES OF MICROBIOLOGY ¾í: 198 ÆÚ: 6 Ò³: 559-564 DOI: 10.1007/s00203-016-1218-4 ³ö°æÄê: AUG 2016 ²é¿´ÆÚ¿¯ÐÅÏ¢ ÕªÒª A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterium, designated LAM0415(T), was isolated from an alcohol fermentation pit mud sample collected from Sichuan Luzhou-flavour liquor enterprise in China. The isolate was found to be able to grow at NaCl concentrations of 0-10 % (w/v) (optimum: 1.0 %), 10-50 A degrees C (optimum: 30-35 A degrees C) and pH 3.0-10.0 (optimum: 7.0-8.0). Phylogenetic analysis of 16S rRNA gene sequences indicated that the new isolate belonged to the genus Bacillus and was closely related to Bacillus sporothermodurans DSM 10599(T) and Bacillus oleronius DSM 9356(T), with 98.4 and 97.2 % sequence similarity, respectively. The DNA-DNA hybridization values between strain LAM0415(T) and the two reference strains were 33.3 +/- A 1.2 and 42.8 +/- A 0.8 %, respectively. The genomic DNA G+C content was 35.2 mol% as determined by the T (m) method. The major fatty acids were determined to be iso-C-15:0, anteiso-C-15:0 and anteiso-C-17:0. The predominant menaquinones were identified as MK7 and MK8. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and four unidentified glycolipids. The diagnostic amino acid of the cell wall peptidoglycan was determined to be meso-diaminopimelic acid. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM0415(T) (=ACCC 06413(T) = JCM 19841(T)) represents the type strain of a novel species of the genus Bacillus, for which the name Bacillus vini sp. nov. is proposed. ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê:Bacillus vini sp nov.; Polyphasic taxonomy; 16S rRNA gene; Pit mud; Alcohol fermentation KeyWords Plus:LUZHOU-FLAVOR LIQUOR; GEN. NOV.; DEOXYRIBONUCLEIC-ACID; RENATURATION RATES; DNA HYBRIDIZATION; SPOROTHERMODURANS; DIVERSITY; OLERONIUS; BACTERIA ×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Ruan, ZY (ͨѶ×÷Õß) ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ CAAS, Key Lab Microbial Resources Collect & Preservat, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China. µØÖ·: ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ [ 1 ] Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ [ 2 ] CAAS, Key Lab Microbial Resources Collect & Preservat, Inst Agr Resources & Reg Planning, Beijing 100081, Peoples R China ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ [ 3 ] Huazhong Agr Univ, Coll Life Sci & Technol, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China [ 4 ] Biogas Inst Minist Agr, Biomass Energy Technol Res Ctr, Chengdu 610041, Peoples R China ÏÔʾÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ [ 5 ] Chinese Acad Fishery Sci, Beijing 100141, Peoples R China µç×ÓÓʼþµØÖ·:zhaobingqiang@caas.cn; ruanzhiyong@caas.cn »ù½ð×ÊÖúÖÂл »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ Key Laboratory of Microbial Resources Collection and Preservation (Ministry of Agriculture, MOA) Special Fund for Agro-scientific Research in the Public Interest 201403019 National Nonprofit Institute Research Grant of CAAS 2014-30 National Key Technology R&D Program of China 2013BAD05B04F02 ²é¿´»ù½ð×ÊÖúÐÅÏ¢ ³ö°æÉÌ SPRINGER, 233 SPRING ST, NEW YORK, NY 10013 USA Àà±ð / ·ÖÀà Ñо¿·½Ïò:Microbiology Web of Science Àà±ð:Microbiology ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000379157100008 PubMed ID: 27055557 ISSN: 0302-8933 eISSN: 1432-072X ÆÚ¿¯ÐÅÏ¢ Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports® ÆäËûÐÅÏ¢ IDS ºÅ: DQ4EQ Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 33 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 0 |
2Â¥2016-09-24 15:22:59
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