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18242016312

新虫 (小有名气)

[求助] 蛋白纯化 已有2人参与

依次未诱导的,诱导的,纯化的。用50mM咪唑漂洗,400mM咪唑洗脱。结果纯化两条带,有什么办法把那条杂带去掉?

蛋白纯化


@biostar2009 发自小木虫Android客户端
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18242016312

新虫 (小有名气)

引用回帖:
2楼: Originally posted by yuy111 at 2016-09-07 00:50:55
The extra band may be the expression protein degradation. Try western blotting and see if the degradation during protein purification.
If it is not try different purification method. No more informa ...

Thanks

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3楼2016-09-07 10:43:36
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yuy111

银虫 (正式写手)


【答案】应助回帖


感谢参与,应助指数 +1
xn8008: 金币+1, 应助指数+1, 欢迎发帖交流 2016-09-07 07:41:16
The extra band may be the expression protein degradation. Try western blotting and see if the degradation during protein purification.
If it is not try different purification method. No more information from your project. Can not tell more.
2楼2016-09-07 00:50:55
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18242016312

新虫 (小有名气)

引用回帖:
2楼: Originally posted by yuy111 at 2016-09-07 00:50:55
The extra band may be the expression protein degradation. Try western blotting and see if the degradation during protein purification.
If it is not try different purification method. No more informa ...

如果真的存在蛋白降解的可能,那怎么办?还能如何纯化这个蛋白,谢谢!

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4楼2016-09-07 13:52:43
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shadow_liu

新虫 (初入文坛)

【答案】应助回帖

感谢参与,应助指数 +1
再过个离子交换柱试试吧,取峰的时候,尽量少取两管试试
5楼2016-09-08 17:11:59
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