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静待花开23

金虫 (正式写手)

嘻嘻哈哈

[求助] 转运体体外实验(HEK293细胞的培养和转染) 已有2人参与

1.HEK293细胞和293T细胞有啥不一样?
2.针对这两种细胞做瞬时转染,选用什么转染方法?钙转法就够了么?有哪些注意事项或者有相关的protocol么?

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zw0123456789zw

至尊木虫 (职业作家)

Felix Baumgartner Space Jump

【答案】应助回帖

感谢参与,应助指数 +1
293:
http://www.atcc.org/SearchCatalogs/longview.cfm?view=ce,913138,CRL-1573&text=CRL%2D1573&max=20
The 293 cell line is a permanent line of primary human embryonal kidney transformed by sheared human adenovirus type 5 (Ad 5) DNA.
The cells express the transforming gene of adenovirus 5.

293T:
http://www.atcc.org/SearchCatalogs/longview.cfm?view=ce,585784,CRL-11268&text=293&max=20
293T cells were cloned and co-transfected with the pBND and pZAP vectors to obtain the G418 resistant 293T/17 (ATCC CRL-11268) cell line.
These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.
2楼2016-08-21 21:28:09
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静待花开23

金虫 (正式写手)

嘻嘻哈哈

引用回帖:
2楼: Originally posted by zw0123456789zw at 2016-08-21 21:28:09
293:
http://www.atcc.org/SearchCatalogs/longview.cfm?view=ce,913138,CRL-1573&text=CRL%2D1573&max=20
The 293 cell line is a permanent line of primary human embryonal kidney transformed by s ...

打开什么也没有
在静好的岁月里做自己喜欢的事
3楼2016-08-22 09:18:07
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zw0123456789zw

至尊木虫 (职业作家)

Felix Baumgartner Space Jump

【答案】应助回帖

★
静待花开23: 金币+1, ★有帮助, 感谢您费心 2016-08-22 15:04:38
看看我写的帖子,能否帮到你。
http://muchong.com/bbs/viewthread.php?tid=10511459
4楼2016-08-22 09:23:04
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静待花开23

金虫 (正式写手)

嘻嘻哈哈

引用回帖:
4楼: Originally posted by zw0123456789zw at 2016-08-22 09:23:04
看看我写的帖子,能否帮到你。
http://muchong.com/bbs/viewthread.php?tid=10511459

已阅,和网上的差不多,还是谢谢你解答
在静好的岁月里做自己喜欢的事
5楼2016-08-22 15:03:54
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engreen2016

禁虫 (正式写手)

★ ★ ★ ★ ★
静待花开23: 金币+5, ★有帮助, 谢谢 2016-09-12 19:49:40
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6楼2016-09-08 17:11:50
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