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kuaile1010新虫 (小有名气)
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[求助]
蛋白冻存
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购买的蛋白的产品说明书中保存方法如下，那分装保存在 -20°C时，我还需要添加甘油之类的吗？顺便问一下，大家冻存蛋白是用哪一种冻存管感觉比较好？ Reconstitution Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C. Physical form Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization. 性质 recombinant expressed in HEK 293 cells assay ≥90% (SDS-PAGE) form lyophilized mol wt calculated mol wt 50.1 kDa observed mol wt 66-90 kDa by SDS-PAGE (DTT-reduced. Asp68 & Tyr19 is the predicted N-terminus .) impurities <1 EU/μg endotoxin (LAL test) conjugate 6-His tagged (C-terminus) UniProt accession no. O15123 shipped in wet ice storage temp. −20°C Gene Information human ... ANGPT1(285) |
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