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专业: 元素化学

[交流] Mol Plant:华中农业大学严建兵发表玉米基因研究成果已有3人参与

2016年7月4日，国际著名学术杂志《Cell》子刊《Molecular Plant》杂志在线发表了华中农业大学生命科学技术学院严建兵教授课题组的叶片研究论文，论文揭示出在玉米遥远的表达数量性状位点(eQTL)和非编码序列对调控基因表达和数量性状变异中起至关重要的作用。严建兵课题组的刘海军和罗欣为论文共同第一作者，严建兵教授为论文通讯作者。
研究人员通过结合简化基因组测序(GBS)、高密度阵列技术，和以往来自368个不同玉米自交系的深度RNA测序数据，鉴别出了次要等位基因频率(MAF)大于0.05的1.25M SNPs。在较大且不同的自然样本中平衡的等位基因频率和分布，帮助鉴别出了与1.8万多个基因(63.4%的测试基因)相关的eQTLs。他们发现在整个基因组中遥远的eQTLs更常见(约75%的eQTLs)。利用这一新数据集，研究人员鉴别出了13个影响玉米粒油浓度的新相关位点。其中，一个基因位点通过控制其他三个已知的油相关基因的表达影响了粒油变化。研究为扩展对玉米基因组内转录组变异细胞调控机制和功能性变异景观的了解，由此增进对数量性状变异的认识提供了重要的资源。
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原文摘要：
A detailed understanding of the genetic architecture of mRNA expression by millions of genetic variants is important for studying quantitative trait variation. In this study, we identified 1.25M SNPs with a minor allele frequency (MAF) greater than 0.05 by combining reduced genome sequencing (GBS), high-density array technologies (600K), and previous deep RNA-sequencing data from 368 diverse inbred lines of maize. The balanced alleleic frequencies and distributions in a relatively large and diverse natural panel helped to identify eQTLs associated with more than 18,000 genes (63.4% of tested genes). We found that distant eQTLs were more frequent (～75% of all eQTLs) across the whole genome. Thirteen novel associated loci affecting the maize kernel oil concentration were identified using the new dataset. Among which, one intergenic locus could affect the kernel oil variation by controlling expression of three other known oil related genes. Altogether, this study provided resources for expanding our understanding of the cellular regulatory mechanisms of transcriptome variation and the landscape of functional variants within the maize genome, thereby enhancing the understanding of quantitative variations.

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