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应助: 1 (幼儿园)
金币: 94.1
红花: 8
帖子: 306
在线: 68.5小时
虫号: 4532612
注册: 2016-03-23
专业: 高分子合成化学

[交流] 采用DSC测试特定生物分子的结合能已有1人参与

We describe here a method using differential scanning calorimetry (DSC) to determine the energy of biomolecular halogen bonds (X-bonds) in a DNA system. This system is unique because we are able to isolate the energetics of a specific molecular interaction from the thermodynamic properties of an entire system. To achieve this goal, we engineered DNA Holliday junctions that are stabilized by either an X-bond or a hydrogen bond (H-bond); the thermodynamic stability of these Holliday junctions are measured through DSC melting studies. Stabilizing energies of the competing X- and H-bonds were determined outside of all of the other interactions by subtracting the energies of these junctions. Overall, the X-bond was shown to have a greater stabilizing potential than the H-bond in this DNA junction system.

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