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Endocannabinoid Signaling: Methods and Protocols
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Endocannabinoid Signaling: Methods and Protocols Methods in Molecular Biology, Book 1412 by Mauro Maccarrone Humana 2016 ISBN: 1493935372 Contents Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix 1 Need for Methods to Investigate Endocannabinoid Signaling . . . . . . . . . . . . . 1 Mauro Maccarrone 2 Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues . . . . . . . . . . . . . . . . . . 9 Laura Bindila and Beat Lutz 3 Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS . . . . . . . . . . . 19 Natalia Battista and Manuel Sergi 4 Analysis of Omega-3 Fatty Acid Derived N -Acylethanolamines in Biological Matrices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27 Renger F. Witkamp and Michiel G. J. Balvers 5 Assay of CB1 Receptor Binding . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41 Valeria M. Catani and Valeria Gasperi 6 The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57 Maria Grazia Cascio , Pietro Marini , and Roger G. Pertwee 7 Assay of TRPV1 Receptor Signaling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 Aniello Schiano Moriello and Luciano De Petrocellis 8 A Functional Assay for GPR55: Envision Protocol. . . . . . . . . . . . . . . . . . . . . . 77 Sharon Anavi-Goffer and Ruth A. Ross 9 The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Pietro Marini , Maria Grazia Cascio , and Roger G. Pertwee 10 Assay of GTPγS Binding in Autoradiography. . . . . . . . . . . . . . . . . . . . . . . . . . 95 Marina Gabaglio , Pamela Prini , Erica Zamberletti , Tiziana Rubino , and Daniela Parolaro 11 Protocol to Study β-Arrestin Recruitment by CB1 and CB2 Cannabinoid Receptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103 Marjolein Soethoudt , Noortje van Gils , Mario van der Stelt , and Laura H. Heitman 12 Assay of NAT Activity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 Toru Uyama and Natsuo Ueda 13 Assay of NAPE-PLD Activity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123 Filomena Fezza , Nicolina Mastrangelo , and Mauro Maccarrone 14 Assay of FAAH Activity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131 Monica Bari , Monica Feole , and Mauro Maccarrone 15 Assay of NAAA Activity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137 Kazuhito Tsuboi and Natsuo Ueda 16 Assay of DAGLα/β Activity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149 Tiziana Bisogno 17 Assay of Monoacylglycerol Lipase Activity . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157 Kwang-Mook Jung and Daniele Piomelli 18 A Sensitive and Versatile Fluorescent Activity Assay for ABHD6 . . . . . . . . . . . 169 Juha R. Savinainen , Dina Navia-Paldanius , and Jarmo T. Laitinen 19 A Sensitive and Versatile Fluorescent Activity Assay for ABHD12 . . . . . . . . . . 179 Juha R. Savinainen , Dina Navia-Paldanius , and Jarmo T. Laitinen 20 Assay of Endocannabinoid Uptake . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 191 Mark Rau , Simon Nicolussi , Andrea Chicca , and Jürg Gertsch 21 Assay of Endocannabinoid Oxidation by Cyclooxygenase-2 . . . . . . . . . . . . . . . 205 Shalley N. Kudalkar , Philip J. Kingsley , and Lawrence J. Marnett 22 Oxygenation of Anandamide by Lipoxygenases . . . . . . . . . . . . . . . . . . . . . . . . 217 Guus van Zadelhoff and Mario van der Stelt 23 Assay of Endocannabinoid Oxidation by Cytochrome P450 . . . . . . . . . . . . . . 227 Natasha T. Snider , Vyvyca J. Walker , and Paul F. Hollenberg 24 Assessing Gene Expression of the Endocannabinoid System. . . . . . . . . . . . . . . 237 Mariangela Pucci and Claudio D’Addario 25 Western Blotting of the Endocannabinoid System . . . . . . . . . . . . . . . . . . . . . . 247 Jim Wager-Miller and Ken Mackie 26 Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255 Jim Wager-Miller and Ken Mackie 27 Measuring ECS Interaction with Biomembranes . . . . . . . . . . . . . . . . . . . . . . . 267 Clotilde B. Angelucci , Annalaura Sabatucci , and Enrico Dainese 28 Visualization of Endocannabinoids in the Cell. . . . . . . . . . . . . . . . . . . . . . . . . 277 Sergio Oddi , Antonio Totaro , and Mauro Maccarrone Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283  |
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2016-06-08 20:28
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