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[资源] Endocannabinoid Signaling: Methods and Protocols

Endocannabinoid Signaling: Methods and Protocols
Methods in Molecular Biology, Book 1412
by Mauro Maccarrone
Humana
2016
ISBN: 1493935372

Contents
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
1 Need for Methods to Investigate Endocannabinoid Signaling . . . . . . . . . . . . . 1
Mauro Maccarrone
2 Extraction and Simultaneous Quantification of Endocannabinoids
and Endocannabinoid-Like Lipids in Biological Tissues . . . . . . . . . . . . . . . . . . 9
Laura Bindila and Beat Lutz
3 Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS . . . . . . . . . . . 19
Natalia Battista and Manuel Sergi
4 Analysis of Omega-3 Fatty Acid Derived N -Acylethanolamines
in Biological Matrices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
Renger F. Witkamp and Michiel G. J. Balvers
5 Assay of CB1 Receptor Binding . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
Valeria M. Catani and Valeria Gasperi
6 The Displacement Binding Assay Using Human Cannabinoid CB2
Receptor-Transfected Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Maria Grazia Cascio , Pietro Marini , and Roger G. Pertwee
7 Assay of TRPV1 Receptor Signaling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
Aniello Schiano Moriello and Luciano De Petrocellis
8 A Functional Assay for GPR55: Envision Protocol. . . . . . . . . . . . . . . . . . . . . . 77
Sharon Anavi-Goffer and Ruth A. Ross
9 The Cyclic AMP Assay Using Human Cannabinoid CB2
Receptor-Transfected Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Pietro Marini , Maria Grazia Cascio , and Roger G. Pertwee
10 Assay of GTPγS Binding in Autoradiography. . . . . . . . . . . . . . . . . . . . . . . . . . 95
Marina Gabaglio , Pamela Prini , Erica Zamberletti , Tiziana Rubino ,
and Daniela Parolaro
11 Protocol to Study β-Arrestin Recruitment by CB1 and CB2
Cannabinoid Receptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
Marjolein Soethoudt , Noortje van Gils , Mario van der Stelt ,
and Laura H. Heitman
12 Assay of NAT Activity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113
Toru Uyama and Natsuo Ueda
13 Assay of NAPE-PLD Activity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Filomena Fezza , Nicolina Mastrangelo , and Mauro Maccarrone
14 Assay of FAAH Activity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 131
Monica Bari , Monica Feole , and Mauro Maccarrone
15 Assay of NAAA Activity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
Kazuhito Tsuboi and Natsuo Ueda
16 Assay of DAGLα/β Activity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
Tiziana Bisogno
17 Assay of Monoacylglycerol Lipase Activity . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157
Kwang-Mook Jung and Daniele Piomelli
18 A Sensitive and Versatile Fluorescent Activity Assay for ABHD6 . . . . . . . . . . . 169
Juha R. Savinainen , Dina Navia-Paldanius , and Jarmo T. Laitinen
19 A Sensitive and Versatile Fluorescent Activity Assay for ABHD12 . . . . . . . . . . 179
Juha R. Savinainen , Dina Navia-Paldanius , and Jarmo T. Laitinen
20 Assay of Endocannabinoid Uptake . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 191
Mark Rau , Simon Nicolussi , Andrea Chicca , and Jürg Gertsch
21 Assay of Endocannabinoid Oxidation by Cyclooxygenase-2 . . . . . . . . . . . . . . . 205
Shalley N. Kudalkar , Philip J. Kingsley , and Lawrence J. Marnett
22 Oxygenation of Anandamide by Lipoxygenases . . . . . . . . . . . . . . . . . . . . . . . . 217
Guus van Zadelhoff and Mario van der Stelt
23 Assay of Endocannabinoid Oxidation by Cytochrome P450 . . . . . . . . . . . . . . 227
Natasha T. Snider , Vyvyca J. Walker , and Paul F. Hollenberg
24 Assessing Gene Expression of the Endocannabinoid System. . . . . . . . . . . . . . . 237
Mariangela Pucci and Claudio D’Addario
25 Western Blotting of the Endocannabinoid System . . . . . . . . . . . . . . . . . . . . . . 247
Jim Wager-Miller and Ken Mackie
26 Quantitation of Plasma Membrane (G Protein-Coupled) Receptor
Trafficking in Cultured Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
Jim Wager-Miller and Ken Mackie
27 Measuring ECS Interaction with Biomembranes . . . . . . . . . . . . . . . . . . . . . . . 267
Clotilde B. Angelucci , Annalaura Sabatucci , and Enrico Dainese
28 Visualization of Endocannabinoids in the Cell. . . . . . . . . . . . . . . . . . . . . . . . . 277
Sergio Oddi , Antonio Totaro , and Mauro Maccarrone
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283