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[×ÊÔ´] Type-1 Diabetes: Methods and Protocols

Type-1 Diabetes: Methods and Protocols
Editors: Gillespie, Kathleen M. (Ed.)
Humana Press
July 5, 2016
ISBN-10: 1493936417

Contents
Preface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v
Contributors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ix
Type 1 Diabetes: Current Perspectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Aizhan Kozhakhmetova and Kathleen M. Gillespie
PARTI GENES
Type 1 Diabetes High-Risk HLA Class II Determination by Polymerase Chain Reaction Sequence-Specific Primers . . . . . . . . . . . . . . . . . . . . . . 13
Rachel J. Aitken, Georgina L. Mortimer, and Kathleen M. Gillespie Pancreatic Beta Cell Survival and Signaling Pathways:
Effects of Type 1 Diabetes-Associated Genetic Variants. . . . . . . . . . . . . . . . . . . . . . . . . 21
Izortze Santin, Reinaldo S. Dos Santos, and Decio L. Eizirik
PART II PREDICTION
Islet Autoantibody Analysis: Radioimmunoassays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Rebecca Wyatt and Alistair J.K. Williams
Islet Autoantibody Detection by Electrochemiluminescence (ECL) Assay. . . . . . . . . 85
Liping Yu
Detection of C-Peptide in Urine as a Measure of Ongoing Beta Cell Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
T.J. McDonald and M.H. Perry
PART III IMMUNOPATHOGENESIS
Histology of Type 1 Diabetes Pancreas . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
Abby Willcox and Kathleen M. Gillespie
Identification of Islet Antigen-Specific CD8 T Cells Using MHCI-Peptide Tetramer Reagents in the Non Obese Diabetic (NOD) Mouse Model
of Type 1 Diabetes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
James A. Pearson and F. Susan Wong
Tracking Immunological Responses of Islet Antigen-Specific T Cells in the Nonobese Diabetic (NOD) Mouse Model of Type 1 Diabetes. . . . . . . . . . . . . 127
Terri C. Thayer and F. Susan Wong
Adoptive Transfer of Autoimmune Diabetes Using Immunodeficient
Nonobese Diabetic (NOD) Mice . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
Evy De Leenheer and F. Susan Wong
PART IV EVOLVING METHODOLOGIES
Methylation Analysis in Distinct Immune Cell Subsets in Type 1 Diabetes . . . . . . . . 143
Mary N. Dang, Claire M. Bradford, Paolo Pozzilli, and R. David Leslie
Fluorescence In Situ Hybridization with Concomitant Immunofluorescence
in Human Pancreas . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 153
Jody Ye and Kathleen M. Gillespie
Laser Capture and Single Cell Genotyping from Frozen Tissue Sections . . . . . . . . . . 159
Thomas Kroneis, Jody Ye, and Kathleen Gillespie
The Gut Microbiome in the NOD Mouse . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
Jian Peng, Youjia Hu, F. Susan Wong, and Li Wen
Molecular Methods and Protein Synthesis for Definition of Autoantibody
Epitopes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
Karen T. Elvers and Alistair J.K. Williams
Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 209

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