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ÎÄÕÂͶ¸å£¬Éó¸åÈËÓиöÒâ¼û²»ÖªµÀÔõô»Ø´ð¡£ Comment #1: The preparation of PBS buffer is not at all clear. The concentration of PBS should express and represent the concentration of phosphate ion! The addition of other salt must be separately reported as electrolyte concentration contained by the buffer. ÎÒÎÄÕÂÖеÄÃèÊöÊÇÕâÑùµÄ£ºPhosphate-buffered solution (PBS) (pH 7.4, 0.01 M) containing 14 mM KH2PO4, 2.7 mM KCl, 87 mM Na2HPO4 and 136.7 mM NaCl was used as the working buffer. лл´ó¼Ò£¬Çë¸÷λ´óÉñ°ï°ï棡 |
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