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lcycomcn铁杆木虫 (著名写手)
千年虫
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[求助]
TEM方法求助
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请各位高手看看图片，是做蛋白的TEM。主要是后面的一半不懂， For rotary shadowing, sample proteins were suspended in solutions of 50% glycerol containing 0.4 M ammonium acetate, pH 7.2. Sample suspensions were sprayed on mica sheets and then the platinum–carbon was shadowed, while rotating with an electron beam evaporator (JFD-9010, JOEL, Tokyo, Japan). Following these treatments, the replica was floated off on water and picked up on a copper grid. Samples were observed under a transmission electron microscope. 有知道麻烦告知！重谢！  TEM.jpg |
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