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| Determining the thermodynamic parameters of a protein by differential scanning calorimetry (DSC) using the CSC Nano-DSC III requires about the same amount of protein as surface plasmon resonance or fluorescence studies. Because of the Nano-DSC III¡¯s extreme sensitivity and baseline reproducibility, and the sample cell¡¯s small volume (300 ¦ÌL) and capillary configuration (which delays the onset of irreversible protein aggregation and precipitation), a complete, interpretable, accurate scan can be obtained on essentially any protein of interest. |
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