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liutieshitao

金虫 (小有名气)

[求助] 关于一篇脂质体文献

文献里面,关于脂质体的体外稳定性,涉及三个方面的实验(1 血清稳定性 2 凝胶渗透 3 体外肿瘤渗透),其中2凝胶渗透我不太懂,外什么要设计这个实验啊?凝胶渗透和体外稳定性有什么关系么?哪位大神能帮我解释一下啊,谢谢了。
它具体的实验步骤如下:For gel permeation, 50 μL of leaky HPPH liposomes (D:L molar ratio
1:4, Dox 3.5 mg/ml), stable std. liposomes (D:L molar ratio 1:4, Dox 3.5
mg/ml) or an equal amount of free Dox were incubated in 500 μl of 50%
bovineserumfor 24 h at 37 °C. A 30% acrylamide solution wasprepared
with Acryl/Bis 19:1 premixed powder (Amresco # 0729); 0.05% ammo-
nium persulfate and 0.05% TEMED were added and the solution was
poured into 3.5 cm diameter petri dishes. Gel was polymerized over-
night. 50 μL of sample was added to a hole in the center of the gel.
Dox fluorescence was imaged with a LUMINA IVIS imager immediately
and after 6 h of incubation at room temperature, using 465 nm excita-
tion and a DS-red emission filter.
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genhunter

至尊木虫 (著名写手)

【答案】应助回帖

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liutieshitao: 金币+50, ★★★★★最佳答案, 多谢多谢 2016-04-20 14:35:39
脂质体个头太大是渗透不出凝胶的。作者想用聚丙烯酰氨凝胶扩散方法来测量药物从两种脂质体中泄漏出来的速度差异。
离成功还差一点
2楼2016-04-19 20:24:08
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