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DSC表征膜蛋白和配体的结合
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| Like ITC, DSC is a valuable approach for studying binding between a biological macromolecule and a ligand such as another biopolymer or a drug. Unlike ITC, DSC allows the thermodynamics that drive binding to be correlated, at least to a degree, with conformational changes in the macromolecule caused by the binding reaction. DSC is particularly useful for characterizing very tight or slow binding interactions. DSC also allows characterization of binding reactions that are incompatible with the organic solvent requirements of some ITC experiments (i.e., where ligand solubility for an ITC experiment requires concentrations of organic solvent not tolerated by the protein). |
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2016-03-31 16:49:06, 1.74 M
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