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让她降落铜虫 (正式写手)
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[求助]
求一段关于细菌胞内DNA以及总DNA提取的完整翻译
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| In particular, DNA was extracted from bacterial colonies picked up from plates, and resuspended in 1.0mL of sterile water up to obtain 10的9次方 harvest cells useful for DNA extraction. Cellular density of water bacterial suspensions was spectroscopically measured (three replicates) by optical density at 600nm. TotalDNA (e.g., genetic material of live bacteria and/or released from inactivated bacteria) was extracted from UV/H2O2 treated watersamples collected at each time. Aliquots (1.0 mL) of each water sample were centrifuged for 10min at 14,000rpm in order to collect and concentrate genetic material up 0.25 OD (600nm). Pellets were resuspended in 180.0 μL of extraction buffer (ATL) and used for DNA extraction. |
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