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| Effects of feeding different available nitrogen sources (corn steep liquor, yeast extract, and ammonium sulphate) in pelluromin biosynthesis phase on the pelluromin A (Pr-A) production were investigated in 50 l fermenter. Under different rate of feeding corn steep liquor and yeast extract from 80 h, the cell growth had no obviously increased, and the biosynthesis had no been inhibited. The Pr-A production was enhanced compared with the control, while the biotransformation from pelluromin C (Pr-C) to Pr-A had no increased. When ammonium sulphate was fed at high feeding rate of 0.04 g/l per hour from 80 h, the cell density kept constant increase and the pelluromin biosynthesis had no negative effect. The maximal Pr-A production was 7953 U/ml at 184 h, which was increased by 17.9% with respect to the control. |
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Effects of feeding different available nitrogen sources (corn steep liquor, yeast extract, and ammonium sulphate) in pelluromin biosynthesis phase on the pelluromin A (Pr-A) production were investigated in 50 L fermenter. Under different rates of feeding corn steep liquor and yeast extract in 80 h, the cell growth rate had no obvious increase, and the biosynthesis was not inhibited. The Pr-A production was enhanced compared with the control, while the biotransformation from pelluromin C (Pr-C) to Pr-A had no increase. When ammonium sulphate was fed at a high feeding rate of 0.04 g/L per hour in 80 h, the cell density constantly increases and the pelluromin biosynthesis had no negative effect. The maximal Pr-A production was 7953 U/ml at 184 h, which was increased by 17.9% with respect to the control. 另外，pelluromin查不到，是不是写错了。 |
2楼2008-10-22 10:23:21