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The bidirectional promoter site between the rebG and rebO genes was replaced with a TRP1 bidirectional promoter cassette and the unidirec- tional promoter in the upstream region of the rebR gene was replaced with a MET15 cassette in which only one promoter was incorporated into the amplicon used for recombination (i.e., aunidirectional promoter cassette) (SI Appendix). |
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